Transmission Electron Microscopes
Krios G3i Cryo-TEM for Life Sciences
Unravelling ‘Life’ at the Molecular Level – Easier, Faster, and more Reliably
The new Thermo Scientific™ Krios™ G3i Cryo Transmission Electron Microscope (Cryo-TEM) enables life science researchers to unravel life at the molecular level—easier, faster, and more reliably than ever before. Its highly stable 300 kV TEM platform and industry-leading Autoloader (cryogenic sample manipulation robot) are designed for automated applications, such as single particle analysis (SPA) and cryo-tomography. Designed-in connectivity ensures a robust and risk-free pathway throughout the entire workflow, from sample preparation and optimization to image acquisition and data processing.
Setting up data acquisition has been made easier and quicker by enhanced automation and systematic user guidance. This allows every user to achieve the ultimate performance for every experiment. Simultaneously, the high-resolution performance and throughput of the Krios G3i Cryo-TEM have been further improved by cleverly combining hardware improvements with advanced software capabilities.
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Key Benefits
Enhanced ease of use through automation
The Krios G3i Cryo-TEM reduces the number of complex alignments that a user has to perform. For recurring alignments that need to be done regularly, automated alignment routines have been implemented into the EPU software for SPA. Furthermore the EPU user interface has been further streamlined to provide comprehensive user guidance.
Reproducible, optimal tool performance always guarantee
The optimal thermal and mechanical stability of the Krios G3i Cryo-TEM ensure perfect optical performance. The instrument features a self-assessment function that automatically evaluates the optical status of the microscope, providing feedback for any steps that require optimization. Additionally, automated alignment routines allow the instrument to be tuned to its optimal starting point for SPA or tomography experiments.
Maximum Throughput
EPU is the native software package for SPA automated screening and acquisition of large data sets on the Thermo Fisher cryo-TEMs. With full control of the Autoloader from within EPU, all grids in a cassette can be batch-screened: after the creation of a grid atlas, ice quality (presence, thickness) of the vitrified grids is automatically categorized to support guided selection of grid squares.
High resolution performance
The Krios Cryo-TEM has a proven track record of high resolution imaging of a wide variety of particles: the vast majority of published structures at or below 4 Å have been determined using Thermo Fisher cryo-TEMs . Constant power lenses reduce thermal drift, and contribute to the excellent system stability during long automated acquisition sessions. To allow imaging of increasingly smaller particles at increasingly higher resolution, the Krios G3i comes with Volta Phase Plate integration and a guaranteed <1% anisotropic magnification distortion.
Workflow connectivity
For successful cryo-EM data acquisition, optimization of both biochemistry and vitrification requires an efficient screening process. The Krios G3i Cryo-TEM can be integrated in a SPA workflow to where samples will be evaluated and optimized using the Talos Arctica or Glacios Cryo-TEMs, before imaging them at high-resolution in the Krios G3i Cryo-TEM
The designed-in connectivity of the Autoloader capsule and cassette systems ensure a robust and contamination-free transfer of samples between Glacios, Arctica and Krios Cryo-TEMs, without the need for manipulation of individual small grids.
Featured Document
Krios G3i Cryo-TEM Datasheet
Our newest Cryo-TEM enables life science researchers to unravel life at the molecular level. Its highly stable 300 kV TEM platform and industry-leading Autoloader (cryogenic sample manipulation robot) are designed for automated applications, such as single particle analysis (SPA) and cryo-tomography. Designed-in connectivity ensures a robust and risk-free pathway throughout the entire workflow, from sample preparation and optimization to image acquisition and data processing.
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Featured Document
Titan Krios TEM Publications
List of published articles and research results with the Titan Krios
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Featured News
Congratulations to the winners of the 2017 Nobel Prize in Chemistry.
Three scientists; Dr. Jacques Dubochet Dr. Joachim Frank, and Dr. Richard Henderson, were awarded the prize for their developments within Cryo-Electron Microscopy.
We are extremely proud of what these researchers and the structural biology community have achieved.
Featured Video
Webcast: Understanding molecular structures – faster and more reliably with cryo-EM
In this webcast, we demonstrate the advantages and applications of the new Thermo Scientific™ Glacios™ and Krios™ G3i Cryo-TEMs for acquiring the 3D structure of molecules. This webcast is specifically tailored for those researchers who are interested in the growing field of cryo-EM imaging in the field of single particle analysis (SPA).
Publication list for Titan Krios for Life Sciences
1. A. Heuer, M. Gerovac, C. Schmidt, S. Trowitzsch, A. Preis, P. Kötter, O. Berninghausen, T. Becker, R. Beckmann, R. Tampé (2017) Structure of the 40S-ABCE1 post-splitting complex in ribosome recycling and translation initiation. Nature structural & molecular biology DOI: 10.1038/nsmb.3396
2.K. Škubník, J. Nováček, T. Füzik, A. Přidal, R. Paxton, P. Plevka (2017) Structure of deformed wing virus, a major honey bee pathogen. Proceedings of the National Academy of Sciences of the United States of America 114 DOI: 10.1073/pnas.1615695114
3.C. Engel, T. Gubbey, S. Neyer, S. Sainsbury, C. Oberthuer, C. Baejen, C. Bernecky, P. Cramer (2017) Structural Basis of RNA Polymerase I Transcription Initiation. Cell 169
DOI: 10.1016/j.cell.2017.03.003
4.J. Gu, M. Wu, R. Guo, K. Yan, J. Lei, N. Gao, M. Yang (2016) The architecture of the mammalian respirasome. Nature 537 DOI: 10.1038/nature19359
