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        Home >Products> Consumables | Lab Wares >Cell / Tissue Culture> 96well Responsive Plate
        Consumables | Lab Wares:  Cell / Tissue Culture   Filtration / Chromatography / Hybridization   Tubes   Pipette Tips   Other Consumables / Lab Wares  
        96well Responsive Plate
        96well Responsive Plate
        Brand:
        Cosmo Biosciences Inc.
        Model:
        12 well /96 well plate,35mm dish
        Updated:
        4/20/2022
          
        | |
        • Product Detail
          Applications
          · Ideal for harvesting fragile cells such as stem cells,neurons etc.
          · Collection of intact and viable single cell suspension.
          · Stem cell culture and tissue engineering culture.
          · Research on extra cellular matrix.
          Feature
          · Made of special polymer Material·
          · Temperature responsive polymer is fixed to the surface of the cultureware at nano-thickness level.
          · Cells are cultured at 37°C and collectable approximately 10-30min after controlling temeperature to 20-25°C.
          Description
          · Collection of intact viable cells without enzymatic processing.
          · Trypsin is well known for its deterioration to the functions of cells. Maintenance of their activities is possible with Cosmo’s
          advanced culture ware is used for cell culture.
          · Harvest cells by mere reduction of temperature.
          · It completely do without trypsin when collecting cells since cell detachs by just the culture surface response.So that the
          Processing would save time 80%.
          · 96 multiwell format is especially suitable for cell cloning by limited dilution.

          Ordering Information

          Cat. No.

          Name

          Description

          2101-035

          35mm Culture Dish

          20/Column

          2101-135

          35mm Culture Dish

          100/Case

          1102-096

          Responses Cell Culture Plate 96well

          10/pack

          1102-196

          Responses Cell Culture Plate 96well

          50/pack



          Instructions Culturing cells
          · First of all, pre-warm the medium to 37°C in the incubator.
          · When seeding cells, please do use the pre-warmed medium to ease attachment of cells.
          · Seed cells evenly over the culture surface to prevent cells being concentrated in one area.
          · The surface becomes hydrophobic above 32°C therefore cells must be cultured above this point.
          · Medium change can be carried out as if using normal culture ware without polymer. Please use pre-warmed medium for change.
          · When cells are difficult to attach, please use medium that contains FCS.
          · Attachment time of the cells may vary depending on the type of the cell.
          Detachment of cells
          · The surface of the cultureware becomes hydrophilic under 32°C. Cells are easier to detach under this temperature.
          · When collecting cells, lower the temperature down to approximately 20°C and wait about 30 minutes. Depending on the type of the cells cultured, they detach automatically.
          · Same results can be achieved by leaving the cultureware in the refrigerator set at 4 degrees celsius for 10 minutes.
          · The cells that did not come off automatically can be detached by agitating them by pipet with medium.
          · Some cells may not detach completely. The results may vary by the type of the cells.
          · Detachment of cells are easier when the temperature is lowered before cells become confluent.
          · The product is intended for one time use only. Please do not re-use the product.
          · The product is only for laboratory use.
          About more advanced responses cell culture ware details , please don't hesitate to contact us:
          E-mail : techservicecosmobrand.com.cn
          Cosmo was founded in 2004, an expert CDMO of sterile and non sterile for life science and medical devices. we own certified class 7 cleanroom and operated by ISO 13485 and GMP regulation.


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