Sequencing Grade Carboxypeptidase B
Catalog Number:SRCPB01
CAS:9025-24-5
EC:3.4.17.2
Storage temperature: 2-8℃
Source: Sequencing grade recombinant carboxypeptidase B is a genetically engineered protein expressed in E.coli
Protein Sequence:The amino acid sequence of recombinant carboxypeptidase B is identical to rat carboxypeptidase B
1.DESCRIPTION
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the optimum pH is 7.5-9.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA.
2. MAIN FEATURES
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Source
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Recombinant E. coli
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Appearance
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White or off white, or yellowish powder
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Specific activity
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≥200 units/mg pro.
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SDS-PAGE
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Single major band
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Molecular Weight(SDS-PAGE)
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33.8±3.4 kDa
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Purity(HPLC)
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≥ 95%
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3.RECOMMEND USAGE
Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7.5-9.0.
4.STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Sequencing grade carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container.It is stable within 24 months.
After dissolved with sterile water or 25mM Tris-HCl, it should be stored under -20℃.
Avoid repeated freezing and thawing.
Stability of transport: The product is stable by blue ice insulation transport.
5.PRODUCT ADVANTAGE
- Sequence grade recombinant protein: high specificity,good stability.
- Animal origin free: Sequencing grade carboxypeptidase B is no exogenous virus contamination,and any animal origin material is not used in the production process.
- Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
- High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
- Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
- Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2008 quality system and GMP guidelines.
- Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.
6.PRODUCT USAGE
1) Determination of protein C-terminal basic amino acids such as arginine, lysine and histidine.
2) detection of antibody basic peak.
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