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- iMatrix Recombinant Laminin E8 Fragments
- Product Detail
- Enables efficient bulk proliferation
- Simple single cell passaging
- Xeno-free recombinant manufacture – regulatory-friendly
- Enhanced cell attachment
- Allows coating free method – saving time and money
iMatrix Recombinant Laminin E8 Fragments
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High Efficient ES/iPS Cell Culture AMSBIO announces iMatrix recombinant Laminin-511 E8 fragments, an innovative cell culture matrix compatible with a wide variety of cell types, and exceptionally well-suited for pluripotent stem cells. Benefits: |
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Miyazaki, T. et al. (2017) Efficient Adhesion Culture of Human Pluripotent
Stem Cells Using Laminin Fragments in an Uncoated Manner. Scientific Reports 7: 41165.
Figure 1. Human iPS cells on laminin-511 E8 (coated laminin concentration 0.4 mkg(micrograms)/cm²):
ZO-1, Beta-Actin and Objective lens; X40)
Figure 2. The Binding activity of Laminin 511 E8 Fragment against ES cell was better than
Full length 511 and traditional substrate. The same function was confirmed in human iPS cells.
Figure 3. E8 fragment contains integrin binding site
Visit our feature on 3D and Physiological Cell Culture
| Laminin-511 is a major component of the basement membrane, which is expressed in early development of the embryo and can be used as a matrix for pluripotent (ES/iPS) stem cells, as it binds to integrin on cell surfaces. However, Laminin-511 is a large protein (800kDa) composed of three chains (alpha, beta and gamma), making it difficult to produce recombinantly. In order to overcome this challenge, Laminin-511 proteins were fragmented to find the smallest integrin-binding component and hES cells were found to adhere more strongly to the E8 fragment than to the full-length protein. |
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AMSBIO’s mission is to be a profitable premier provider of quality life science research reagents and services helping customers develop innovative methods, processes, products and medicines. This is achieved by offering small and medium size manufacturers, academic groups and revenue generating biotechs a unique partnership for the global market and by providing state of the art and cost effective solutions to end users and partners.
Formed in 1987 by Sandy Allan and Alex Sim the company’s first offices were in Madrid, Spain. Over the following three years further offices were opened in Italy, Switzerland and the UK. During the formative years of existence AMS helped launch the successful sales campaigns of Stratagene and Invitrogen providing them with an important foothold from which to develop their global brand expansion.
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