產(chǎn)品編號(hào) 9603-400-B
OrganoPlate 2泳道400具有96個(gè)立的培養(yǎng)細(xì)胞，每個(gè)細(xì)胞支持一個(gè)凝膠內(nèi)培養(yǎng)和灌注通道，可以包含灌注的管狀組織。車道寬400微米。384孔板用戶界面的四個(gè)孔連接到每個(gè)微流體網(wǎng)絡(luò)。
mimetas-三維器官芯片OrganoPlate? 2-lane、OrganoPlate? 3-lane、The OrganoPlate? Perfusion Rocker? Mini
型號(hào):9603-400-B、4003-400-B
聯(lián)系人:程園
聯(lián)系電話:18611717737
品牌:mimetas
MIMETAS：Organ-on-a-Chip （器官芯片）公司介紹
讓復(fù)雜的組織研究變得j為簡(jiǎn)單
于為復(fù)雜的3D生物學(xué)創(chuàng)造簡(jiǎn)單的設(shè)備和裝置。通過(guò)對(duì)較容易控制的灌注血管，共培養(yǎng)和化微環(huán)境的使用，以至于你不會(huì)察覺(jué)到自己使用的裝置可能是的組織培養(yǎng)平臺(tái)。通過(guò)OrganoPlate®這些愿景都能實(shí)現(xiàn)。OrganoPlate® 是3D組織模式的未來(lái)。
顯而易見(jiàn)社會(huì)需要更好的藥品。而為了達(dá)到這一點(diǎn)，需要在充分考慮人類和生理上相關(guān)性的基礎(chǔ)上，研發(fā)更好的疾病模型。作為真正的信息工具，需要在兼容化合物的同時(shí)和還具有高通量讀出功能。作為organ-on-a-chip生物學(xué)真正的先驅(qū)者，本公司可開(kāi)展任何通量的人體組織和疾病模型相關(guān)的OrganoPlate®產(chǎn)業(yè)。
OrganoPlate®是一種基于微流體技術(shù)3D細(xì)胞培養(yǎng)板，可在一塊板上實(shí)現(xiàn)96個(gè)單組織模型的培養(yǎng)。Phaseguides?能夠以3D方式j(luò)確地重新定義細(xì)胞及其培養(yǎng)基，實(shí)現(xiàn)細(xì)胞間的相互作用以及前所未有品質(zhì)的成像和定量操作。
OrganoPlate® 2-lane
圖1：單個(gè)培養(yǎng)細(xì)胞的微流體設(shè)計(jì) 圖2：2車道和3車道OrganoPlate的特點(diǎn)和應(yīng)用Supported tissue models
The 2-lane OrganoPlate® is a microfluidic 3D culture plate, supporting up to 96 tissue models on a single plate.
The OrganoPlate contains Phaseguides?, a proprietary, passive liquid handling technology for membrane-free definition of tissues in microfluidic chambers.
The OrganoPlate is based on the industry-standard 384?well plate for full compatibility with liquid handling- and readout equipment.
The OrganoPlate 2-lane 400 supports a range of cell types, including neurons, endothelial tubules, hepatocytes, CaCo2 cells, organoids and kidney epithelial proximal tubules.
This plate allows for culture in-gel, tubular culture against the gel and combinations of in-gel and tubular culture.
What is a culture cell in the OrganoPlate®?
A culture cell is a fully autonomous organ-on-a-chip culture unit in the OrganoPlate®.
The OrganoPlate 3-lane contains 40 3-lane culture cells, the 2-lane 96 2-lane culture cells, all in a standard 384 well plate format.
The culture cells on a single OrganoPlate may be used all at once in an experiment, but can also be used individually.
What is a lane in an OrganoPlate® culture cell?
A lane is an individually addressable part of a culture cell in the OrganoPlate®. The OrganoPlate 3-lane features 3 adjacent lanes per culture cell, 2 of which are perfusable.
The OrganoPlate 2-lane features 2 adjacent lanes per culture cells, of which 1 is perfusable. A single lane may contain cells in an ECM gel, a perfused epithelial tubule or just medium flow.
By virtue of patented Phaseguide technology, no physical barriers are present between the lanes in the OrganoPlate, allowing co-cultured cells in adjacent lanes to interact and migrate freely.
Materials and equipment
The materials and equipment you need to work with the OrganoPlate® 2-lane 400 are listed below.
- Cells (cell lines, primary cells, iPSC-derived cells and others)
- Medium and standard culture materials
- Extracellular Matrix Gel, for example Collagen I or Matrigel® (Corning®)
- Mimetas Perfusion Rocker? Mini (optional, please refer to the section below)
- Pipetting reservoirs 50 ml (e.g. VWR no. 613-1183)
- Multichannel pipette 5-350 ?L (e.g. Sartorius® no. 730361)
- Multichannel tips (e.g. Sartorius® 790351)
- Eppendorf® Repeater® M4 Dispenser (Eppendorf® no. 4982000322)
- Eppendorf® Combitips advanced® pipette tips Biopur® grade, 0.1 ml
- Sartorius® eLINE® Electronic Pipette for volumes between 0.2-10 ?L (Sartorius® no. 730021)
- Sartorius® 0.1-10 ?L tips (Sartorius® no. 790010)
We will provide you with detailed instructions for use, including materials and equipment.
OrganoPlate ® 3車道
產(chǎn)品編號(hào) 4003-400-B
OrganoPlate 3通道具有40個(gè)立的培養(yǎng)細(xì)胞，每個(gè)細(xì)胞支持相鄰的凝膠內(nèi)培養(yǎng)和2個(gè)灌注通道，可以包含灌注的管狀組織。3通道模型支持上皮和內(nèi)皮小管的頂端和基底通路，允許屏障完整性和運(yùn)輸測(cè)定。
圖1：單個(gè)培養(yǎng)細(xì)胞的微流體設(shè)計(jì) 圖2：2車道和3車道OrganoPlate的特點(diǎn)和應(yīng)用
車道寬400微米。384孔板用戶界面的六個(gè)孔連接到9x9孔網(wǎng)格中的每個(gè)微流體網(wǎng)絡(luò)，中心孔提供對(duì)微組織的觀察訪問(wèn)。
Supported tissue models
The OrganoPlate® 3-lane is a microfluidic 3D culture plate, supporting up to 40 tissue models on a single plate. The OrganoPlate contains Phaseguides?, a proprietary, passive liquid handling technology for membrane-free definition of tissues in microfluidic chambers. The OrganoPlate is based on the industry-standard 384?well plate for full compatibility with liquid handling- and readout equipment.
The OrganoPlate 3-lane 400 supports a range of cell types, including neurons, endothelial tubules, hepatocytes, CaCo2 cells, organoids and kidney epithelial proximal tubules. This plate allows for culture in-gel, tubular culture against the gel and combinations of in-gel and tubular culture in 3 lanes, allowing cells to interact through the absence of separating membranes or barriers.
Read this for more information on tissue model design in the OrganoPlate 3-lane 400.
What is a culture cell in the OrganoPlate®?
A culture cell is a fully autonomous organ-on-a-chip culture unit in the OrganoPlate®. The OrganoPlate 3-lane contains 40 3-lane culture cells, a 2-lane 96 2-lane culture cells, all in a standard 384 well plate format. The culture cells on a single OrganoPlate may be used all at once in an experiment, but can also be used individually.
What is a lane in an OrganoPlate® culture cell?
A lane is an individually addressable part of a culture cell in the OrganoPlate®. A 3-lane OrganoPlate features 3 adjacent lanes per culture cell, of which 2 are perfusable. The OrganoPlate 2-lane features 2 adjacent lanes per culture cells, of which 1 is perfusable. A single lane may contain cells in an ECM gel, a perfused epithelial tubule or just medium flow. By virtue of patented Phaseguide technology, no physical barriers are present between the lanes in the OrganoPlate, allowing co-cultured cells in adjacent lanes to interact and migrate freely.
Materials and equipment
The materials and equipment you need to work with the OrganoPlate® 2-lane 400 are listed below.
- Cells (cell lines, primary cells, iPSC-derived cells and others)
- Medium and standard culture materials
- Extracellular Matrix Gel, for example Collagen I or Matrigel® (Corning®)
- Mimetas Perfusion Rocker? Mini (optional, please refer to the section below)
- Pipetting reservoirs 50 ml (e.g. VWR no. 613-1183)
- Multichannel pipette 5-350 ?L (e.g. Sartorius® no. 730361)
- Multichannel tips (e.g. Sartorius® 790351)
- Eppendorf® Repeater® M4 Dispenser (Eppendorf® no. 4982000322)
- Eppendorf® Combitips advanced® pipette tips Biopur® grade, 0.1 ml
- Sartorius® eLINE® Electronic Pipette for volumes between 0.2-10 ?L (Sartorius® no. 730021)
- Sartorius® 0.1-10 ?L tips (Sartorius® no. 790010)
We will provide you with detailed instructions for use, including materials and equipment.
Perfusion Rocker Mini
The Perfusion Rocker? Mini provides a continuous medium flow in the OrganoPlate® with minimal settings required. Perfusion Rockers? feature optimized angles, time settings and programs for use with the OrganoPlate®, in contrast to ordinary continuous see-saw rockers.
Operation
Medium perfusion (flow) in the OrganoPlate® is established by passive leveling of liquids. The perfusion can be maintained indefinitely by placing the OrganoPlate® on a MIMETAS Perfusion Rocker?. This customized rocker platform fits easily in an incubator and enables interval rocking (switching sides every 5-15 minutes), as well as adjustable rocking angles.
Minimal settings are required for pump- and tube-free perfusion in OrganoPlates®. Perfusion Rockers? feature optimized time- and angle settings and programs for use with OrganoPlates®. The Perfusion Rocker comes in two versions: Perfusion Rocker? Mini for 2 stacks of plates and the Perfusion Rocker?Maxi for 4 stacks of plates. Both versions are suitable for use in standard tissue culture incubators, the Perfusion Rocker? Maxi is available on request.
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