FMP微圖案多孔板和微圖案皿-世聯(lián)博研國(guó)總
型號(hào):Micropatterns
聯(lián)系人:陳寶華
聯(lián)系電話:13060393900
品牌:4d cell
微圖案載片、35mm培養(yǎng)皿、多孔板-世聯(lián)博研quan國(guó)代理
將細(xì)胞培養(yǎng)在具有預(yù)定義幾何特征的微米級(jí)圖案化的基質(zhì)中。當(dāng)在其上進(jìn)行培養(yǎng)時(shí)，細(xì)胞會(huì)te異地附著在圖案區(qū)域上，從而獲得例如圖案的幾何形狀。
不同的共價(jià)結(jié)合表面化學(xué)性質(zhì)（圖案壽命，細(xì)胞性質(zhì)）：
標(biāo)準(zhǔn)抗粘聚合物
抗氧化抗粘聚合物
長(zhǎng)期細(xì)胞培養(yǎng)抗粘 聚合物
不同的圖案形狀和尺寸（線，正方形，三角形，網(wǎng)格等）
適用于任何細(xì)胞培養(yǎng)底物（從培養(yǎng)皿到384孔板）
與高分辨率光學(xué)顯微鏡系統(tǒng)兼容
應(yīng)用領(lǐng)域
細(xì)胞形狀控制和標(biāo)準(zhǔn)化，細(xì)胞定位的特征，細(xì)胞成熟和分化，遷移區(qū)域的限制等
寬度為100至10 um的行中的HeLa細(xì)胞
應(yīng)用示例
肝小管化驗(yàn)：改進(jìn)的肝細(xì)胞分化模型
神經(jīng)網(wǎng)絡(luò)：控制神經(jīng)元連通性
附加球體測(cè)定法：改進(jìn)了對(duì)球體的操作和觀察
心肌成熟試驗(yàn)：功能性IPSc誘導(dǎo)的心肌纖維
細(xì)胞j化：細(xì)胞 形狀和組織的標(biāo)準(zhǔn)化
初次纖毛測(cè)定：通過細(xì)胞形狀控制纖毛生成
微圖案多孔板
微圖案多孔板可容納旨在組織細(xì)胞正?；?strong>完美組織的培養(yǎng)底物。細(xì)胞均勻分布在膠粘劑圖案上，具有可控的幾何形狀，可實(shí)現(xiàn)化驗(yàn)的標(biāo)準(zhǔn)化。
微圖案技術(shù)可實(shí)現(xiàn)2D單元幾何形狀控制。一組圖案布置在玻璃底部以容納細(xì)胞。使用抗粘劑和某些ECM蛋白（例如纖連蛋白或膠原蛋白），細(xì)胞可以粘附到微圖案所強(qiáng)加的形狀。單元將采用這種新環(huán)境的幾何結(jié)構(gòu)。
FMP micropatterning TM是一塊96孔板，其表面具有微圖案，可控制細(xì)胞的2D幾何形狀并標(biāo)準(zhǔn)化細(xì)胞測(cè)定。
每個(gè)96孔板均具有6種標(biāo)準(zhǔn)形狀：點(diǎn)，正方形，矩形，三角形，直線和網(wǎng)格，尺寸從10到150 um。我們還提供可定制解決方案的選擇。
FMP micropatterning TM與篩選平臺(tái)的工業(yè)標(biāo)準(zhǔn)兼容。
FMP板的底部由玻璃制成，并涂有防粘聚合物以劃定圖案的邊界。該涂層具有抗氧化性，使其足以在幾天內(nèi)將電池電鍍?cè)趫D案上。
標(biāo)準(zhǔn)FMP微型圖案
FMP
Micropatterning TM具有廣泛的應(yīng)用范圍。研究和測(cè)量：
癌癥
·
癌細(xì)胞遷移（線型）
器官生理學(xué)
·
體細(xì)胞和癌細(xì)胞的遷移
·
像元形狀標(biāo)準(zhǔn)化
·
心肌搏動(dòng)特性
·
神經(jīng)網(wǎng)絡(luò)
罕見疾病
·
細(xì)胞核完整性
·
核可塑性
·
神經(jīng)網(wǎng)絡(luò)
基礎(chǔ)研究
·
像元形狀標(biāo)準(zhǔn)化
·
神經(jīng)元之間的標(biāo)準(zhǔn)化連接
體外生物分子模型
·
大分子的空間自組織
（微觀）
微圖案皿
微圖案化的幻燈片是印有微圖案的24毫米玻璃幻燈片。
它們已經(jīng)準(zhǔn)備好使用，您只需要將細(xì)胞鋪在玻璃基板上，并在te定的微圖案上觀察它們即可。
提供的標(biāo)準(zhǔn)形狀是圓盤，直線，正方形，矩形，三角形和網(wǎng)格。
您也可以要求我們?yōu)槟O(shè)計(jì)的定制形狀。
二、MICROPATTERNED MULTIWELL PLATES 96 OR 384 WELLS
For high throughput screening and high content imaging of cell-based micropatterned assays
micropatterned multi-well plates hold a perfectly organized and stable culture substrate aiming for cell normalization. Evenly distributed on adhesive patterns, cells have a controlled geometry allowing standardization of the assays.
micropatterning technology enables 2D cell geometry control. A set of patterns is arranged on a glass bottom to receive cells. Using an anti-adhesive agent and some ECM proteins such as fibronectin or collagen, cells adhere and acquire the shape imposed by the micropattern.
> IMAGING OF SINGLE CELLS AND ORGANIZED CELL GROUPS
> ROBOT PIPETTE READY / SBS 96 OR 384-WELL PLATES
> HIGH-RESOLUTION IMAGING
> COMPATIBLE WITH IMAGING SOFTWARE COMMERCIALLY AVAILABLE
> MINIMAL AUTO-FLUORESCENCE
HOW TO HANDLE YOUR 96 OR 384 MICROPATTERNED WELL PLATES
FEATURES & BENEFITS
> micropatterned 96 well plates hold a patterned surface to control the 2D geometry of cells and standardize cellular assays.
> Each 96-well plate has the 6 standard shapes of : dots, squares, rectangles, triangles, lines, and grids, as well as sizes going from 10 to 150 ?m.
> We also offer the choice of a customizable solution (additional shapes and dimensions).
> The device is compatible with industrial standards for screening platforms (e.g. drug discovery).
> The bottom of the plate is made in glass and is coated with a stable anti-adhesive polymer to delimitate the borders of the patterns. This coating is resistant to oxidation, can be kept at room temperature for six months, and enables keeping the cells in patterns for several days.
Map of the standard Micropatterned 96 well plate
DRUG SCREENING
> High throughput and high content screening
ORGAN PHYSIOLOGY & DISEASE
> Migration of somatic and cancer cells
> Cell shape standardization
> Cardiomyocyte beating properties
> Neural network and neuromuscular junction assays
> Cell nucleus integrity
> Nuclear plasticity
> Co-culture
> Wound healing
> Cellular division
> Cell-cell contact and interaction
> Cell polarization
> Frustrated phagocytosis
> Cellular membrane protrusion assays (lamellipodia and filipodia)
BIOMOLECULAR IN VITRO MODELS
> Spatial auto-organization of macromolecules and organelles (at the microscale)
>
三、DYNAMIC MICROPATTERNED COVERSLIPS，動(dòng)態(tài)微圖案載片
Patterned slides ready to be plated with cells for cell migration assays
微圖案培養(yǎng)皿是35毫米玻璃底培養(yǎng)皿，上面印有微圖案。 它們已經(jīng)準(zhǔn)備好使用，您只需要將細(xì)胞鋪在玻璃基板上，等待它們被圖案化，然后觀察它們?cè)诨迳线w移的情況。
>即用型
將細(xì)胞直接鋪在帶圖案的蓋玻片上，并直接觀察
>高品質(zhì)的細(xì)胞成像
通過光學(xué)透明的微圖案玻璃基板觀察細(xì)胞
>非常穩(wěn)定的涂層
在防粘涂層和玻璃蓋玻片之間形成的共價(jià)鍵可在室溫下保存幾個(gè)月
>可能通過雙正交化學(xué)法制成的涂層
shou先在細(xì)胞粘附區(qū)域中將細(xì)胞圖案化，并且不粘附在這些區(qū)域的外部。 通過將一種試劑添加到基材上，可以使周圍的抗粘連區(qū)域具有粘性：這會(huì)誘導(dǎo)細(xì)胞遷移到新的粘連區(qū)域。
HOW TO HANDLE YOUR ‘DYNAMIC’ MICROPATTERNED COVERSLIPS
The micropatterned dishes are provided in individual sterile bags (aseptic conditions). Available in sets of 20 or 50 dishes to allow optimization oy your experimental conditions with only one kit.
Compatible with high resolution microscopy, phase contrast, immunofluorescence, etc.
The coverslips may be combined with a perfusion chamber.
MICROPATTERNS STANDARD SHAPES AVAILABLE:
As with regular micropatterned coverslips and dishes, the available shapes are:
Disks
Lines
Triangles
Squares
Rectangles
Grids
Customized shapes
Each type of micropatterned coverslip (disks, rectangles, squares, etc.) is arranged as described in the scheme bellow (e.g.: disks).
In each standard coverslip, there are seven zones with distinct pattern sizes (ranging from 10 ?m to 100 ?m). There is an eighth zone where all the sizes are placed together.
Standard positionning of micropattern areas
A HIGHLY STABLE NON-ADHESIVE COVALENT COATING
Long term cell culture anti-adhesive polymer which is the best in terms of anti-fouling treatment and bonding to the glass slide. Observable by phase contrast microscopy. This is the best option for long term cell culture on micropatterns (more than a week of cell culture).
A HIGHLY EFFICIENT REAGENT FOR RENDERING THE COATING ADHESIVE
he reagent for switching the coating from ‘non-adhesive’ to ‘ adhesive’ and activate cell migration is shipped in aliquots of 50 ug lyophilized powder. Once dissolved in its appropriate solvent, it can be stored at -20°C.
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