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Recreating the Tumor Microenvironment on a Chip SynVivo-Tumor is a microfluidic 3D cell-based assay platform for quantitative assessment in a physiologically realistic tumor microenvironment. The system enables real time visualization and analysis of cell-cell and cell-drug interactions encompassing (a) circulation in the microvasculature, (b) transport across the vessel walls, and (c) delivery to the tumors. [...]

SynVivo is announcing the availability of a new assay chip designed specifically to provide a long term cell culturing environment compatible with advanced microscopy systems from?CytoViva. CytoViva’s technology combines patented, enhanced darkfield optical microscopy with high resolution hyperspectral imaging. These integrated technologies enable label free optical observation and spectral characterization of nano-materials as they interact [...]

Researchers at Oregon State University have developed a new three-drug delivery system for cancer treatment, especially metastatic melanoma, the deadliest form of skin cancer - and shown that the system may have particular value with cancers like this that often spread through the lymphatic system. The new technology takes advantage of nanoparticles that can migrate [...]

Researchers at Temple University used the?SynVivo? cell-based?in vitro?assay platform to model the attributes and functions of the neonatal stage blood-brain barrier (BBB) [1]. The SynVivo BBB model closely mimics the?in vivo?microenvironment including three-dimensional morphology, cellular interactions and flow characteristics on a microfluidic chip.?This work marks the first dynamic?in vitro?neonatal BBB model that offers real time [...]

Realistic and Dynamic In Vitro Model of the Blood Brain Barrier Traditional blood brain barrier (BBB) assays, such as the Transwell? model have significant limitations such as lack of physiological shear stress, real-time visualization capability and large amount of consumables in addition to cumbersome protocols. SynVivo-BBB system recreates the in vivo micro-environment by emulating a [...]

Adding a New Dimension to Your Migration Assays Use of Transwells for migration experiments have shown that these static assays are difficult to use, often have repeatability issues, and do not provide as much quantitative data as desired. Imagine an assay which is repeatable, simple to use, provides real-time live cell imaging and gives data [...]

A nanopolymer used for drug delivery was complexed with GFP DNA and perfused on tumor cell (HeLa) cultured networks. GFP transfection, efficiencies and distribution was quantified at 24 hours post injection. HeLa Cells Transfection with GFP Gene Outcome: SynVivo can support the growth of tumor cells and [...]

Different shapes of particles were obtained and coated with anti-BSA antibody. SynVivo walls were coated with BSA. Particles were injected into SynVivo and the adhesion quantified. SEM Images of Particle Shapes Comparison between Linear Channels and Microvascular Networks Outcome: Linear channel assays cannot differentiate between various shapes, [...]

A confluent layer of endothelial cells were activated with cytokines. Anti-ICAM-1 coated particles were used for targeting (red dots) and IgG particles served as controls (green dots). Data was obtained at 4 and 24 hours post treatment. Time Lapse Image of Confluent Layer of Endothelial Cells [...]

P-selectin, an inflammatory molecule was coated on the walls of SynVivo. Particles coated with the antibody to P-selectin were injected into SynVivo and their differential binding pattern was observed. Particle Adhesion Shear Rate Distribution Shear Rate Map Outcome: SynVivo can be used to quantify particle [...]