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        Home >Products> Reagents >Cell Biology> Enzymatic Chromatrap® Premium Pro-A spin column shearing kit
        Reagents:  Molecular Biology   Biochemistry   Cell Biology   ELISA / Diagnostic Kits   Antibody   Serum/Medium   Other Reagents  
        Enzymatic Chromatrap® Premium Pro-A spin column shearing kit
        Enzymatic Chromatrap® Premium Pro-A spin column shearing kit
        Brand:
        Porvair Sciences Limited
        Model:
        Premium Pro-A
        Updated:
        2/16/2022
          
        • Product Detail

          Product Description

          24 Chromatrap®  Protein-A based spin-columns, buffers and reagents for enzymatic shearing of chromatin for ChIP assays.

          This Premium kit also includes the following reference standards:

          Positive Antibody Control : Histone 3 Rabbit polyclonal
          Negative Antibody Control: IgG from Rabbit serum 0.2µg/µl
          Forward primer: GAPDH (human promoter region)
          Reverse primer: GAPDH (human promoter region)

          Kit Contents:

          Chromatrap® spin columns 24
          Column conditioning buffer 50mls
          Wash Buffer 1  50mls
          Wash Buffer 2  50mls
          Wash Buffer 3 50mls
          1.3M Glycine  20mls
          Lysis Buffer 10mls
          Digestion Buffer 10mls
          Enzymatic Stop Solution 200µl
          0.1M Elution Solution 3mls
          0.1M NaOH  3mls
          5M NaCl 500µl
          1M NaHCO3 500µl
          Hypotonic Buffer  10mls
          Shearing Cocktail 100µl
          Protease Inhibitor Cocktail (PIC) 100µl
          Proteinase K stop solution 100µl
          Proteinase K  50µl
          1.5ml Collection tube 24

          Porvair Sciences, specialists in the manufacture of microplate products, serve Life Sciences, Biotechnology, R&D and Molecular Biology with microplate solutions for all applications, from sample preparation to high throughput screening via our global distributor network.

          Our range includes vacuum manifolds, sealers, evaporators and microtiter plates in all popular styles; deep well and shallow well storage plates, assay plates, luciferase reporter gene plates and liquid handling reagent reservoirs. We also provide custom microplate products for life science research.

          Our vacuum manifolds, essential for 96-well SPE sample preparation, are designed to work with most popular filter plates, including Waters, Millipore, Qiagen, Whatman, GE Healthcare, Varian, Biotage and, of course, Porvair.

          For further information, including custom built microplates, reagent reservoirs, vacuum manifold design or microplate handling equipment, please contact us: Tel: +44 1978 666240; Email: int.salesporvair-sciences.com.
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