The in vitro transcription vector constructed by the company is a simple and effective RNA transcription system, which is suitable for a variety of research. The principle is to obtain mRNA through mRNA in vitro transcription vector, which is widely used in in vitro translation, biochemical research, protein expression after injection into cells or embryos, and other mRNA applications of specific sequences.
The system uses the T7 promoter upstream of the target sequence to efficiently generate mRNA under appropriate reaction conditions through T7 phage RNA polymerase (T7 RNAP) and triphosphate nucleotide. The first two bases at the 3 'end of T7 promoter are GG, followed by the target sequence. In vivo application, the addition of 7-methylguanosine cap at the 5’ end of mRNA is very necessary for RNA stability, effective translation, nuclear transport and splicing. Capped RNA is also necessary for most research applications. RNA capping can be modified by Co-transcription using cap analogues or post transcription using capping enzymes. Both methods can produce functional capped RNA suitable for injection, in vitro translation and other applications.
Advantage
The mRNA in vitro transcription vector constructed can be efficiently used for T7 RNAP mediated in vitro transcription. The vector has high copy replication ability in E. coli, is easy to digest, and is conducive to the production of a large number of mRNA.
High efficiency: T7 RNAP is a highly efficient enzyme, and its mediated transcription in vitro can produce a large number of functional RNA.
Simple technique: the operation is simple and easy, which is easier than expressing mRNA in cells, and the expression of mRNA in cells also needs transfection and cell culture.
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