1.Optical Stretcher

Features：
single cell analysis with laser beams,a novel laser tool to measure and analyse biomechanical properties!The optical stretcher is a novel laser tool to micromanipulate single biological cells and to probe their viscoelastic properties in suspension.Based microfluidic technology for high-throughput analysis of single cell traction and measurement;Automated measurement and imaging properties of single cell force recorded cell deformation record
1)Contact-free cell deformation
Contact-free cell deformation” open=”0″ style=”2″]Suspended cells are deformed by optical forces, leading to absolutely contact-free measurements. This ensures homogeneous cell handling and avoids artefacts due to contact-induced cellular reactions.
2)Timesaving, automated measurements
Cells are automatically delivered to the measurement region and deformed corresponding to the user-defined stretch pattern. While the Optical Stretcher runs the experiment you can focus on interpreting results.
3)High-throughput single cell rheology
By integration of a microfluidic system about 300 cells/hour can be measured with ease. This allows for the first time to collect significant statistical data from cell rheology.
4)Better statistics compared to AFM
A cell is trapped by two opposing laser beams, which hold it and pull on both sides of it. Higher laser powers are used to deform the cell. The cell deformations are recorded by a CCD camera and evaluated by a custom designed software. The measurement chamber of the Optical Stretcher is integrated into a microfluidic system, such that cells can be easily delivered one after the other. High throughput rates of about 250 cells per hour can be achieved, allowing for better statistics compared to other tools such as atomic force microscopy (AFM).

2.Single Molecule Biophysics Magnetic Trap—Stretching and twisting single molecules

Features：
PicoTwist is involved in nanomanipulation and single-molecule experiments in biology. Historically, single molecule experiments were among the first nanotechnologies, appearing in the 1990s to characterize biopolymers and proteins with unprecedented accuracy. The idea is that instead of analyzing the behavior of a great number of molecules as usually done in a test tube, you now analyze the behavior of a single molecule. Thus instead of measuring average behavior you have direct access to the biopolymer’s activity in real time.

3.Chiaro Nanoindenter

Features：
Compatible with microscopes
The Chiaro Nanoindenter is designed to be compatible with almost any inverted microscope to enable the simultaneous practice of advanced microscopy and micro-mechanical characterization.
Features Chiaro Nanoindenter
The Chiaro Nanoindenter is engineered with flexibility in mind. Due to the almost limitless mounting options and low footprint of our nanoindentation probes, the Chiaro can be combined with many microscopes or other optical set-ups, even simultaneous with other instruments such as electrophysiology equipment, patch clamping instruments, perfusion or heating stage chambers, and many more!
Standard versus dynamic
The standard version of the Piuma and Chiaro Nanoindenter includes all the tools required for performing displacement-controlled experiments, including automatically mapping the micro-mechanical properties of surfaces. The Piuma and Chiaro Nanoindenter are available with a dynamic module, expanding the modes of operation with load-, indentation-, and frequency control.

4.Piuma Nanoindenter

The Piuma Nanoindenter is designed to operate as a stand-alone instrument in any lab setting. Amongst many applications possible, the Piuma can be used to perform comparative studies on biological samples such as cartilage, dermis or cornea, optimize and select between polymer or hydrogel synthesis or crosslinking procedures or define material performance parameters such as surface adhesion, swelling or degradation, also in liquids.
Features：
Easy operation due to the built-in camera (top-view, 5X) and the coarse manual Z-stage. Almost any sample size or shape can be used thanks to the large sample stage. Optionally a sample stage heater is available (from room temperature up to 60oC), a back light illumination module, an inverted camera module for small or transparent samples and an acrylic housing for use in more noisy environments.

RegenHU 3Ddiscovery 3D bio-printing platform

The 3DDiscovery® instrument is a cost-effective 3D bio-printing platform to explore the potential of 3D tissue engineering through the bio-printing approach.

RegenHU BioFactory® high end, versatile and cell friendly three dimensional bio-manufacturing instrument

The BioFactory® is a high end, versatile and cell friendly three dimensional bio-manufacturing instrument. It allows researchers to pattern cells, biomolecules and a range of soft and rigid materials in desirable 3D composite structures in order to mimic biomimetic tissue models.

Neuroindx UnipicK vacuum-assisted single cell collection and tissue microdissection

UnipicK is a vacuum-assisted single cell collection and tissue microdissection instrument based on NeuroInDx’s original capillary-based cell and tissue acquisition system (CTAS; USPT 8,797,644; Kudo et al, 2012) that can acquire individual cells from cell cultures and tissues. It permits rapid acquisition of single cells from any untreated cell cultures, providing reliable and affordable means of most sophisticated experimentation. Moreover, the instrument is suitable for individual cell collection and region specific acquisition of subanatomical areas from complex heterogeneous tissues such as the brain. Operation of the system is intuitive and requires minimal training. Cell cultures require no treatment or specific peripherals, thus enabling unmatched flexibility in experimental design and sample collection.
Perhaps one of the most distinct features is the ability of UnipicK™ to collect live cells for further reculturing and a plethora of downstream functional studies. Both individual fluorescently labeled and morphologically distinct cells can be acquired from any cell cultures or tissue sections. The procedure has been demonstrated from nearly all types of adherent, suspended and 3D cultures, including difficult samples such as primary cells, or individual cells from fresh frozen and sucrose treated complex tissues, such as brain (please, see http://www.youtube.com/user/NDXInc). With live cells viability tests have demonstrated up to 99% after collection, and up to 30 cells per minute may be collected from adherent cultures.
Notable Features of UnipicK™ include:
– Simple operation with minimal training
– Single cell collection from untreated cultures and tissue sections
– Efficient tissue microdissection
– High viability of collected cells
– High quality RNA and protein for downstream – omics studies
– Flexibility and versatility – fit any inverted microscope
– Fraction of the cost of any laser assisted microdissection instrument

UnipicK+ tissue microdissection, single cell acquisition, and deposition platform

UnipicK+ is a universal platform for tissue microdissection, single cell acquisition, and deposition into the single wells for further molecular analysis or clonal expansion. The main working principle is based on the capillary based vacuum pulse assisted technology (CTAS; Kudo et al, 2012). Developed system can microdissect tissue sections, collect and deposit individual cells from any adherent cultures grown in standard cell culture dishes in as small as 15 nl volume, compatible with various downstream single cell analyses and next generation sequencing. Like UnipicK, the system may be used with a wide range of inverted microscopes. The benefits include cost-efficiency, simple operation, digital interface, complete workflow from single cell isolation to downstream analysis, compatibility with a wide range of inverted microscopes and use of standard plates and culture dishes.

CellSorter High throughput single cell sorting

High throughput single cell sorting directly from the Petri dish
One single cell arrives to each PCR tube
10 PCR strips containing 80 tubes can be filled in a cycle
Glass cover slip for testing single cell deposition in situ
Drop volume less than 1 ul for adherent cells
Pick up volume of ~1 nl for suspended cells
15-20 seconds per cell. When collecting multiple cells, sorting speed is 1 cell/second.
Number of cells picked up in a single run: 1-1000.
Isolates a subpopulation of live adherent cells expressing fluorescent or luminescent markers
Both unlabeled and fluorescentcells are recognized by computer vision
Viable cells after sorting
Any adherent and non-adherent cell type can be sorted
Cell culture needs minimal preparation before sorting
Average sorting process takes only a few minutes
Multichannel detection using the fluorescent filter setup of the microscope