水凝支架三維培養(yǎng)系統(tǒng)，水凝膠基質(zhì)力學(xué)環(huán)境模擬系統(tǒng)，水凝膠基質(zhì)力學(xué)環(huán)境系統(tǒng)，生物材料細(xì)胞力學(xué)微環(huán)境體外構(gòu)建系統(tǒng)

型號(hào):fx-5000tt
聯(lián)系人:李勝亮
聯(lián)系電話:18618101725
品牌:美國(guó)flexercell

三維種子細(xì)胞構(gòu)建人工生物組織系統(tǒng)(Creating a Bioartifical Construct with the Tissue Train System)

特點(diǎn)：

1）對(duì)生長(zhǎng)在三維狀態(tài)下的細(xì)胞進(jìn)行靜態(tài)的或者周期性的牽張拉伸刺激培養(yǎng)，可以進(jìn)行實(shí)時(shí)觀察分析。

2）對(duì)生長(zhǎng)在三維環(huán)境下的細(xì)胞進(jìn)行單軸向或者雙軸向的靜態(tài)或者周期性的應(yīng)力加載實(shí)驗(yàn)

3)可建立te制的各種模擬實(shí)驗(yàn)：心率模擬實(shí)驗(yàn)，步行模擬實(shí)驗(yàn)，跑動(dòng)模擬實(shí)驗(yàn)和其他動(dòng)力模擬實(shí)驗(yàn)。
4)可構(gòu)建長(zhǎng)度達(dá)35mm的生物人工組織

5）具有豐富的三維培養(yǎng)模具和多種蛋白包被材料的自動(dòng)細(xì)胞組織三維培養(yǎng)系統(tǒng)

6）該系統(tǒng)以立體水凝膠為三維培養(yǎng)支架， 水凝膠支架具有大量體內(nèi)微環(huán)境基質(zhì)的特征，水凝膠所具有的三維網(wǎng)絡(luò)結(jié)構(gòu)、含水量高和力學(xué)性能可控等特性與體內(nèi)細(xì)胞所處基質(zhì)微環(huán)境相似, 被廣泛用于工程化組織的體外構(gòu)建研究，水凝膠的硬度調(diào)控范圍很大, 非常有利于模擬體內(nèi)生理或病理力學(xué)微環(huán)境
是真正意義上的三維培養(yǎng)系統(tǒng)

7）配套的scanflex掃描分析模塊可以記錄三維人工組織中凝膠的壓實(shí)過(guò)程、記錄三維細(xì)胞培養(yǎng)凝膠的壓實(shí)動(dòng)力學(xué)、凝膠面積計(jì)算

An automated scanning device with area measurement software.

• Measure gel compaction in 3D bioartificial tissues.
• Scans and saves images up to 600 dpi of 3D tissue constructs.
• Can be used in conjunction with the Tissue Train^® Culture System.

適用范圍

2水凝膠基質(zhì)力學(xué)環(huán)境模擬

3）生物材料的細(xì)胞力學(xué)微環(huán)境體外構(gòu)建系統(tǒng)

4）基于干細(xì)胞3D力學(xué)環(huán)境的工程化微組織構(gòu)建研究

Tissue Train^® Trapezoidal Construct under Tension with Corresponding Finite Element Strain Values
Trapezoidal-shaped 3D cell-seeded gel construct (created with the flexercell^® Tissue Train^®System) undergoing unconstrained tension applied with the FX-5000? Tension System. The strain values, as determined with Finite Element Analysis, are depicted alongside the strained construct.

Tissue Train ®培養(yǎng)系統(tǒng)應(yīng)用背景

體外培養(yǎng)在與真實(shí)組織在結(jié)構(gòu)上和功能上相似的人工組織需要以下幾個(gè)基本條件：

（1）細(xì)胞

（2）支架矩陣組織

（3）培養(yǎng)基和生長(zhǎng)因子和（4）機(jī)械刺激。這些條件彼此相互影響，并且相互之間共同來(lái)促進(jìn)形成能夠承受生物機(jī)械力的，且結(jié)構(gòu)比較穩(wěn)定的組織。而在人工組成形成的過(guò)程中，這些細(xì)胞按照發(fā)育途徑形成具有一定幾何形狀的細(xì)胞外基質(zhì)結(jié)構(gòu)。其中一些信號(hào)轉(zhuǎn)導(dǎo)途徑參與了細(xì)胞外基質(zhì)組合物的形成。這些途徑中，有些是由細(xì)胞基質(zhì)的機(jī)械變形調(diào)節(jié)，并通過(guò)膜結(jié)合蛋白，如整合素，粘著斑復(fù)合體，細(xì)胞粘附分子和離子通道傳遞到細(xì)胞內(nèi)。這些途徑中細(xì)胞還可以響應(yīng)配體，如細(xì)胞基質(zhì)形變所釋放的細(xì)胞因子，激素或生長(zhǎng)因子等。

為了維持肌肉骨骼組織的完整性和強(qiáng)度，組織內(nèi)細(xì)胞需要保持一定水平的的內(nèi)在應(yīng)力。如果缺乏這種內(nèi)在的應(yīng)力，組織會(huì)缺少?gòu)?qiáng)度導(dǎo)致細(xì)胞結(jié)構(gòu)的破壞或者組織的斷裂。目前一般認(rèn)為如果在固定四肢，臥床休息或在內(nèi)在應(yīng)力水平的降低的情況下，將導(dǎo)致骨中礦物質(zhì)流失，骨組織萎縮，骨骼弱化，以及合成代謝活性的降低和分解代謝活性的增加。

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