PRODUCT NUMBER:

Species Reactivity: Human

Host: Rabbit

Immunogen Source: Human Erythrocyte

Form: IgG fraction from antisera obtained by ammonium sulfate precipitation and DEAE-Sephacel ion-exchange chromatography.

Buffer: phosphate buffered saline with 0.05% NaN3 as a preservative

Storage: +2 to 8°C

Working Dilution: >1:2000 by indirect ELISA using ALPH-conjugated secondary antibody

Western Blot: At 1:16000 dilution, antisera reacts to 0.75, 0.15, and 0.3 ug catalase and to 1 uL of red blood cell lysate using ALPH conjugated secondary antibody.

IEP: Single arc upon immunoelectrophoresis of red blood cell lysate.

The Catalase immunogen used to prepare this antibody was purified in the Athens Research & Technology laboratory. It is a native protein, whole molecule, isolated from human erythrocytes.

PLEASE NOTE: This product is a laboratory reagent and is not to be administered to humans or used for any drug purpose. For research use only.

Ref:
Kim SJ, Cheresh P, Jablonski RP, Morales-Nebreda L, Cheng Y, Hogan E, Yeldandi A, Chi M, Piseaux R, Ridge K, Michael Hart C, Chandel N, Scott Budinger GR, Kamp DW. Mitochondrial catalase overexpressed transgenic mice are protected against lung fibrosis in part via preventing alveolar epithelial cell mitochondrial DNA damage. Free Radic Biol Med. 2016 Dec;101:482-490. doi: 10.1016/j.freeradbiomed.2016.11.007. Epub 2016 Nov 11.

Xiao W and Goswami PC. Down-regulation of peroxisome proliferator activated receptor γ coactivator 1α induces oxidative stress and toxicity of 1-(4-Chlorophenyl)-benzo-2,5-quinone in HaCaT human keratinocytes. Toxicol In Vitro. 2015 Oct;29(7):1332-8. doi: 10.1016/j.tiv.2015.05.009. Epub 2015 May 22.