PRODUCT NUMBER:16-14-051200
MW: 29,500
Extinction Coefficient: 0.985
Salt-free lyophilized solid.
Reconstitution: After initial centrifugation, we suggest the addition of 50 mM Na Acetate, pH 5.5, with 150 mM NaCl to the original volume, followed by gentle swirling and/or vortexing to ensure adequate homogenization.
Storage: -20°C
Buy purified Native Human Elastase direct from the manufacturer.
Same day shipping.
This enzyme degrades elastin, collagen, and proteoglycan and has been implicated in the development of pulmonary emphesema and rheumatoid arthritis.
Ref: Baugh, R.J. and Travis, J. 1976. Biochemistry. 15,836; Bode, W. et Al. 1986. EMBO J. 5 (10), 2453
Activity: 20-22 units per mg protein. One unit is defined as the amount of enzyme that hydrolyzes one umole of MeO-Suc-Ala-Ala-Pro-Val-pNA per minute at 25oC, pH 7.5.
Purity: >=95% by SDS-PAGE
Prepared from whole blood shown to be non reactive for HBsAg, anti-HCV, anti- HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
Athens Research & Technology products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research use only.
Product Citation:
Mittendorf, Elizabeth A., Gheath Alatrash, Na Qiao, Yun Wu, Pariya Sukhumalchandra, Lisa S. St John, Anne V. Philips et al. "Breast cancer cell uptake of the inflammatory mediator neutrophil elastase triggers an anticancer adaptive immune response."?Cancer research?72, no. 13 (2012): 3153-3162
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Edwards, J. Vincent, Nicolette Prevost, Kandan Sethumadhavan, Abul Ullah, and Brian Condon. "Peptide conjugated cellulose nanocrystals with sensitive human neutrophil elastase sensor activity."?Cellulose?(2013): 1-13.
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Progranulin-mediated deficiency of cathepsin D results in FTD and NCL-like phenotypes in neurons derived from FTD patients.
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Neuropilin-1 mediates neutrophil elastase uptake and cross-presentation in breast cancer cells.
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Proteinase 3 Is a Phosphatidylserine-binding Protein That Affects the Production and Function of Microvesicles.
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Neutrophil elastase enhances antigen presentation by upregulating human leukocyte antigen class I expression on tumor cells.
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α-1-Antitrypsin variants and the proteinase/antiproteinase imbalance in chronic obstructive pulmonary disease.
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Miropin, a Novel Bacterial Serpin from the Periodontopathogen Tannerella forsythia, Inhibits a Broad Range of Proteases by Using Different Peptide Bonds within the Reactive Center Loop.
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Leukocyte Protease Binding to Nucleic Acids Promotes Nuclear Localization and Cleavage of Nucleic Acid Binding Proteins.
J Immunol. 2014 Jun 1;192(11):5390-7. doi: 10.4049/jimmunol.1303296. Epub 2014 Apr 25.
Ungurs MJ, Sinden NJ, Stockley RA.
Progranulin is a substrate for neutrophil-elastase and proteinase-3 in the airway and its concentration correlates with mediators of airway inflammation in COPD
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The significance of the F variant of alpha-1-antitrypsin and unique case report of a PiFF homozygote.
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Inhibition of Plasma Kallikrein by a Highly Specific Active Site Blocking Antibody.
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