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PRODUCT NUMBER:

Species Reactivity: Human

Host: Rabbit

Immunogen Source: Human Neutrophil

Form: Ig Fraction (Standard Antisera) obtained by ammonium sulfate precipitation.

Buffer: phosphate buffered saline with 0.05% NaN3 as a preservative

Storage: +2 to 8°C

Protein Concentration: 5.1 mg/mL

Working Dilution: Greater than 1:4000 by indirect ELISA.

Western Blot: At a 1:1000 dilution, reacts to 0.3 ug purified antigen when run under non-reducing conditions. Does not show a reaction when antigen is run under reducing conditions.

IEP: Single arc against human neutrophil granule extract and human neutrophil elastase.

The Elastase immunogen used to prepare this antibody was purified in the Athens Research & Technology laboratory. It is a native protein, whole molecule, isolated from human neutrophils.

PLEASE NOTE: This product is a laboratory reagent and is not to be administered to humans or used for any drug purpose. For research use only.

References:

Skrzeczynska-Moncznik J, et al. Eosinophils regulate interferon alpha production in plasmacytoid dendritic cells stimulated with components of neutrophil extracellular traps. J Interferon Cytokine Res. 2017 Mar;37(3):119-128. doi: 10.1089/jir.2016.0036. Epub 2017 Feb 10.

Zabieglo K, et al. The inhibitory effect of secretory leukocyte protease inhibitor (SLPI) on formation of neutrophil extracellular traps. J Leukoc Biol. 2015 Jul;98(1):99-106. doi: 10.1189/jlb.4AB1114-543R. Epub 2015 Apr 27.