MDM4, encodes a 490-amino acid protein containing a RING finger domain and a putative nuclear localization signal.The MDM4 putative nuclear localization signal, which all Mdm proteins contain, is located in the C-terminal region of the protein.The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested.MDM4 protein produced by in vitro translation interacts with p53 via a binding domain located in the N-terminal region of the MDM4 protein.MDM4 shows significant structural similarity to p53-binding protein MDM2.

Target	MDM4
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Tested Applications	ELISA, WB, IHC, IF/ICC
Recommended dilutions	ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, IF/ICC: 1/200 - 1/1000. Optimal dilutions/concentrations should be determined by the end user.
Immunogen	Purified recombinant fragment of human MDM4 expressed in E. coli.
Purification	Unpurified Ascites.
Isotype	IgG1
Conjugation	Unconjugated
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Molecular Weight	55 kDa
Swiss Prot	O15151
GeneID	4194
Gene Symbol	MDM4
OMIM	602704
HGNC	6974
Ensembl	ENSG00000198625
Buffer	Ascitic fluid containing 0.03% sodium azide.
UNSPSC Code	12352203
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.