| Target |
Preferentially Expressed Antigen In Melanoma (PRAME) |
| Reactivity |
Human |
| Tested Applications |
ELISA |
| Recommended dilutions |
Dilute the Capture Antibody with Coating Buffer.
Dilute the biotin-conjugated Detection Antibody with Detection Antibody Diluent. Optimal dilutions/concentrations should be determined by the end user. |
| Form |
Liquid (Capture Antibody and Detection Antibody) |
| Reconstitution |
Reconstitute the standard with Standard Diluent. The volume, and therefore standard concentration, should be determined by the end user. |
| Storage |
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Standard Form |
Lyophilized |
| ELISA Type |
Sandwich |
| Capture Antibody Conjugation |
Unconjugated |
| Detection Antibody Conjugation |
Biotin |
| Buffer |
The capture and detection antibody both contain 0.1% sodium azide. |
| Directions for use |
Bring all components to room temperature (18-25°C) and briefly spin or centrifuge the vials before use. Working solutions should be prepared and used immediately.
Recommended Procedure: - Dilute the Capture Antibody to working concentration using Coating Buffer. Immediately coat the 96-well plate with diluted Capture Antibody (100 μl per well). Seal the plate and incubate at 4 °C overnight or at 37 °C for 2 hours
- Aspirate the wells and wash with Wash Buffer (350 μl per well) and allow to soak for 1-2 min. Remove the liquid by inverting and tapping the plate on to absorbent paper.
- Block the plate with Blocking Buffer (200 μl per well) at 37 °C for 1.5 hours.
- Repeat the aspiration/wash process in Step 2.
- Add 100 μl of standards or sample into the appropriate wells. Cover with a plate sealer and incubate at 37 °C for 1 hour.
- Repeat the aspiration/wash process in Step 2.
- Add appropriately diluted biotin-conjugated Detection Antibody (100 μl per well). Cover the plate with a new plate sealer and incubate at 37 °C for 1 hour.
- Repeat the aspiration/wash process in Step 2.
- Add appropriately diluted Streptavidin HRP (100 μl per well). Cover the plate with a new plate sealer and incubate at 37 °C for 30 min.
- Repeat the aspiration/wash process in Step 2.
- Add Substrate Solution (90 μl per well). Cover the plate with a new plate sealer and incubate at 37 °C for 10-20 min. Keep the plate in the dark and avoid exposure to light.
- Add Stop Solution (50 μl per well). Tap the side of the plate to ensure thorough mixing.
- Measure the absorbance immediately using a microplate reader set at 450 nm.
|
| Availability |
Shipped within 5-20 working days. |
| Note |
This product is for research use only. |