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Human CD45RA is expressed on all cells of hematopoietic origin, except erythrocytes. CD45RA is a transmembrane tyrosine phosphate which can exist in at least nine different isoforms resulting from tissue-specific alternative RNA splicing of exons 4-7 of a single gene coding for the various N-terminal peptide segments. The CD45RA isoform predominates on naive/resting T cells and medullary thymocytes.

Target	CD45RA
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Tested Applications	FCM
Recommended dilutions	FCM: 20 μl/1 million cells. Optimal dilutions/concentrations should be determined by the end user.
Immunogen	Tissue / cell preparation (Human). Fresh human lymph node lymphocytes depleted of surface immunoglobulin positive cells were used as the immunogen. This antigen is a single chain glycoprotein which is expressed on B cells, monocytes, and T cell subsets.
Purification	Affinity Chromatography
Form	Liquid
Isotype	IgG1
Conjugation	FITC
Specificity	The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4+ peripheral Tlymphocytes, CD8+ peripheral T lymphocytes, and B cells. The CD45RA+ T cell populations are mainly naive/virgin allowing the use of HI100mAb as a phenotypic marker to discriminate T cell subsets.
Storage	Aliquot and store in the dark at 2°C to 8°C. Avoid exposure to light.
Molecular Weight	220 kDa
Swiss Prot	P08575
GeneID	5788
Enzyme Commission Number	EC 3.1.3.4
Buffer	The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium Azide
UNSPSC Code	12352203
Availability	Shipped within 3-5 working days.
Note	This product is for research use only.