Amersham Protran membranes are manufactured from 100% pure nitrocellulose to ensure the highest binding capacity possible. They are compatible with a variety of detection methods, including isotopic, chemiluminescent, colorimetric and fluorescent. No methanol pre-wetting step is required, making this the membrane of choice for proteins which prefer aqueous environments. Prior to transfer the membrane is simply wetted with water, and then placed in the transfer buffer. No other pretreatment steps are necessary.

Amersham Protran offers excellent sensitivity, resolution, and low background, especially when used with Amersham ECL, Amersham ECL Prime, or Amersham ECL Select Western Blotting Systems.

Available in a choice of pore sizes: Protran 0.1 NC (pore size 0,1 μm), Protran 0.2 NC (pore size 0,2 μm), or Protran 0.45 NC (pore size 0,45 μm).

The versatile Protran 0.1 NC membrane (binding capacity 231 to 248 μg IgG/cm2) provides excellent binding affinity for small proteins and peptides, as well as nucleic acids.

Protran 0.2 NC (binding capacity 150 to 176 μg IgG/cm2) binds small proteins (< Mr 20 000).

For convenience, Western blotting sandwiches (10 sandwiches/package) are available, consisting of precut Amersham Protran NC membranes preassembled with 2 × 3MM Chr Blotting papers.