| 產(chǎn)品名稱 | mRNA Cap2'-O-Methyltransferase, New England Biolabs |
| 產(chǎn)品貨號(hào) | M0366S |
| 產(chǎn)品價(jià)格 | 現(xiàn)貨詢價(jià)，電話：010-67529703 |
| 產(chǎn)品規(guī)格 | |
| 產(chǎn)品品牌 | vwr |
| 產(chǎn)品概述 | |
| 產(chǎn)品詳情 |
mRNA Cap 2′-O-Methyltransferase adds a methyl group at the 2′-O position of the first nucleotide adjacent to the cap structure at the 5′ end of the RNA
The cap-1 structure has been reported to enhance mRNA translation efficiency and hence may help improve expression in mRNA transfection and microinjection experiments. mRNA Cap 2′-O-Methyltransferase specifically requires RNA with an m7GpppN cap as substrate. It cannot utilize RNA with pN, ppN, pppN or GpppN at the 5′ end. Capped RNA may be prepared via in vitro transcription using cap analog or through enzymatic capping using the Vaccinia Capping Enzyme.
The enzyme utilizes S-adenosylmethionine (SAM) as a methyl donor to methylate capped RNA (cap-0) resulting in a cap-1 structure.
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