| 產(chǎn)品名稱 | SelecTEV? Protease, Lucigen |
| 產(chǎn)品貨號 | 30810-2 |
| 產(chǎn)品價格 | 現(xiàn)貨詢價，電話：010-67529703 |
| 產(chǎn)品規(guī)格 | |
| 產(chǎn)品品牌 | vwr |
| 產(chǎn)品概述 | |
| 產(chǎn)品詳情 |
SelecTEV? Protease is a 27 kD improved form of Tobacco Etch Virus (TEV) protease that has been engineered to be more stable, active, and specific than the native protease
SelecTEV? Protease can be used with the Expresso? Solubility and Expression Screening System vectors, which contain a TEV cleavage recognition site.
For research use only. Not for human or diagnostic use.
SelecTEV? Protease is a cysteine protease that recognizes the TEV recognition sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Gln and Gly. This seven-amino-acid sequence is rarely found in proteins, making SelecTEV? Protease an ideal choice for tag removal of fusion proteins. The optimal temperature for cleavage is 30 °C, however the enzyme is active over a wide temperature range (4 – 30 °C) and pH (6.0 – 8.5) to accommodate your specific protein. Following digestion, SelecTEV? Protease is easily removed from the cleavage reaction by affinity chromatography using the polyhistidine tag at the N-terminus of the protease. SelecTEV? Protease is purified from E. coli by affinity chromatography using the polyhistidine tag and is 90% pure when visualized on an SDS-PAGE gel.
Ordering information: Kits contain SelecTEV? Protease, 100 mM DTT, and 20X SelecTEV? Buffer
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