Lucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α?, DH10B?, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields, and reliability, while decreasing costs. These cells are unrivalled in quality, efficiency, and exceptionally low costs. Lucigen's E. cloni cells provide solutions for a wide range of applications at remarkably economical prices.

Lucigen offers the following choices of strain: 1) E

And the following choices of efficiency: 1) E. cloni 10G SUPREME Electrocompetent Cells ≥4 x 10¹⁰ cfu/μg pUC. SUPREME Cells have the highest transformation efficiency available from any supplier. Choose SUPREME Cells for the most demanding cloning situations, such as construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible. 2) E. cloni 10G & 10GF′ ELITE Electrocompetent Cells ≥2 x 10¹⁰ cfu/μg pUC. ELITE Cells have twice the transformation efficiency compared to “ultra high efficiency” cells from other suppliers. ELITE Cells provide large numbers of transformants from hard-to-clone fragments or limited DNA at a lower price. 3) E. cloni 10G CLASSIC Electrocompetent Cells ≥5 x 10⁹ cfu/μg pUC. CLASSIC Cells are high efficiency cells with a substantially lower cost per reaction. These cells are the most economical choice for standard cloning and library construction. 10G CLASSIC Cells are available in larger package sizes for convenient use in higher volume cloning applications.

The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

cloni 10G Competent Cells. Library construction, cloning, subcloning, and plasmid isolation with or without blue/white screening. 2) E. cloni 10GF′ Competent Cells. Contain the F' plasmid for infection with M13 to produce ssDNA.