| 產(chǎn)品名稱 | EconoTaq? DNA Polymerase, Lucigen |
| 產(chǎn)品貨號(hào) | 30031-3 |
| 產(chǎn)品價(jià)格 | 現(xiàn)貨詢價(jià)，電話：010-67529703 |
| 產(chǎn)品規(guī)格 | |
| 產(chǎn)品品牌 | vwr |
| 產(chǎn)品概述 | |
| 產(chǎn)品詳情 |
EconoTaq? DNA Polymerase is an excellent Taq polymerase for routine PCR
EconoTaq? DNA Polymerase is a high performance Taq polymerase offered with your choice of reaction buffers, with or without MgCl2. EconoTaq? DNA Polymerase also offers exceedingly high lot-to-lot reproducibility and reliability.
Concentration: 5 units/μl. One unit catalyzes the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 70°C in 50mM Tris-HCl (pH 9.0), 50mM NaCl, 5mM MgCl2, 200μM dGTP, dATP, dTTP dCTP (a mix of unlabeled and [33P] dCTP), 10μg of activated calf thymus DNA, and 0.1mg/ml BSA. Storage Buffer: 10mM Tris-HCl (pH 7.5), 100mM KCl, 0.1% Triton X-100, 0.1mM EDTA, 1mM DTT, and 50% glycerol. 10X Reaction Buffer: 100mM Tris-HCl (pH 9.0), 500mM KCl, 1% Triton X-100, and with or without 15mM MgCl2. PCR Activity: EconoTaq? DNA Polymerase is tested in DNA amplification using a variety of templates and primers.
Activity Determination: One unit of EconoTaq? DNA Polymerase catalyzes the incorporation of 10nmoles of dNTP into acid-insoluble material in 30 minutes at 70°C in 50mM Tris-HCl (pH 9.0), 50mM NaCl, 5mM MgCl2, 200μM dGTP, dATP, dTTP, dCTP (a mix of unlabeled and [33P]dCTP), 10μg Activated Calf Thymus DNA, and 0.1mg/ml BSA. Absence of Endoonuclease or Nicking Activity: Incubation of 10U of EconoTaq? DNA Polymerase with 1μg of supercoiled pBR322 DNA for 16 hours at 70°C results in no detectable conversion to relaxed or linear forms detectable by agarose gel electrophoresis. Absence of Exonuclease Activity: Incubation of 10U of EconoTaq? DNA Polymerase with 1μg of HindIII-cut lambda DNA for 16 hours at 70°C resulted in no smearing of bands on agarose gels. Purity: EconoTaq? DNA Polymerase is >99% pure as determined by SDS PAGE. There is no detectable DNA contamination.
It performs just as well as more expensive alternatives, including Tfl
Ordering information: EconoTaq? DNA Polymerase is provided with a choice of 10X Reaction Buffer with Mg++ (“with Mg++”); or 10X Reaction Buffer without Mg++ and a separate tube of 25mM MgCl2 (“separate Mg++”).
|
| 產(chǎn)品資料 |