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產(chǎn)品名稱

Active Recombinant Human GM-CSF from AGRENVEC

產(chǎn)品貨號

RF0036

產(chǎn)品價格

現(xiàn)貨詢價，電話：010-67529703

產(chǎn)品規(guī)格

1 μg, 10 μg, 20μg, 50μg, 100 μg, 250 μg

產(chǎn)品品牌

AGRENVEC

產(chǎn)品概述

產(chǎn)品詳情

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Product Name	Active Recombinant Human GM-CSF
Description	Human recombinant protein expressed in Nicotiana benthamiana. It is produced by transient expression in non-transgenic plants. This product contains no animal-derived components or impurities. Animal Free product. GMCSF is a cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, such as granulocytes, macrophages, eosinophil's and erythrocytes. Is involved in differentiation of dendritic cells and is a key factor in differentiation pathways leading form stem cells. GMCSF is produced by several cell types as monocytes, fibroblasts, endothelial cells and T- Lymphocytes in response to a number of inflammatory mediators present in the hemopoietic environment and peripheral site of inflammation. Human GMCF is an important therapeutic cytokine used in the treatment of myeloid leukaemia, neutropenia and aplastic anaemia and it could become interesting in the treatment following bone marrow transplantation. It performs biological activity by binding to a specific receptor complex which is composed of a cytokine-specific alpha chain and beta chain shared with the receptors for interleukin-3 and interleukin-5. GMCSR has been identified to mediate the activation of Jak-Stat and MAPK pathways. Recombinant human GM-CSF is a glycosylated polypeptide chain containing 127 amino acids (18-144 aa CSF2_HUMAN P04141 ), fused to 10 His tag at N-terminal. rHuman GM-CSF migrates as a broad band between 15 and 25 kDa due to post-translation modifications, in particular glycosylation. Purity >97% by SDS-PAGE gel
Size	1 μg, 10 μg, 20μg, 50μg, 100 μg, 250 μg
Concentration	n/a
Applications	Functional studies, Cell culture. Western Blot. Immunogen. Dermocosmetics (rh GM-CFS stimulates fibroblast cell proliferation, kerotinocytes migration (HaCaT cells) and induces Collagen I expression in keratinocytes).
Other Names	Colony-stimulating factor
Gene, Accession, CAS #	UniProt: P04141
Catalog #	RF0036
Price
Order / More Info	Active Recombinant Human GM-CSF from AGRENVEC
Product Specific References	- Kitamura, T. et al., 1989. Establishment and characterization of a unique human cell line that proliferates dependently on GMCSF, IL-3 or erythropoietin. J. Cell Phyisiol., 140 (3 ): 323-334. - Goodall G. J. et al., 1993. A model for interaction of the GM-CSF, IL-3 and IL-5 receptors with their ligands. Growth Factors, 8(2): 87-97. Sonoda, Y. et al., 1998. Erytroid burst-promoting activity of purified recombinant human GM-CSF and interleukin-3: studies with anti-GM-CSF and anti-IL-3 sera and studies in serum-free cultures. Blood, Oct; 72 (4): 1381-1386. - Kato T. et al., 2008. Distinct role of c-Jun N- terminal kinase isoforms in human neutrophil apoptosis regulated by tumour necrosis factor alpha and granulocytes-macrophages colony-stimulating factor. J. Interferon Cytokine res. Apr; 28 (4): 253-43. - Hamilton J. A., 2002. GM-CSF in inflammation and autoimmunity. Trends Immunol., Aug; 23(8): 403-8. - Brandt, S. J. et al., 1988. Effect of recombinant human Granulocyte-macrophage colony-stimulating factor on hematopoietic reconstitution after high-Dose Chemotherapy and Autologous Bone Marrow Transplantation. N. Engl. J. Med., 7: 318(14) 869-76. - Stout B. A. et al., 2004. IL-5 and granulocyte-macrophage colony stimulating factor activate STAT 3 and STAT 5 and promote Pim-1 and cyclin D3 protein expression in human eosinophil's. J. Immunol., Nov; 15 173 (10): 6409-6417. - Gurhridge, M. A. et al., 1998. Mechanisms of activation of the GM-CSF, IL-3, and IL-5 family of receptors. Stem Cells, 16: 301-313.
產(chǎn)品資料

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