| Description | Human recombinant protein expressed in Nicotiana benthamiana. It is produced by transient expression in non-transgenic plants. This product contains no animal-derived components or impurities. Animal Free product. Myostatin belongs to the transforming growth factor beta (TGFBs) superfamily, which includes : TGF-betas, Bone morphogenetic protein (BMPs), Growth differentiation factors (GDFs), Activins and Inhibins. As other members of this superfamily, is synthesized and secreted as a homodimeric prepropeptide that is cleaved by proprotein convertases such as furin, to generate the dimeric N- terminal propeptide and the dimeric C-terminal mature active protein. Myostatin is one of the most important protein that controls myoblast proliferation and it is a potent negative regulator of skeletal muscule mass in many animal species. Several studies have shown that Myostatin could play an important role in cardiac development and physiology. Genetic deletion of Myostatin or in vivo administration of the Myostatin propeptide induces muscle hypertrophy as well as enhanced glucose utilization and insulin sensitivity and a reduction in overall fat mass. Recombinant human Myostatin is a homodimer polypeptide chain containing 2X 109 amino acids (267 - 375 of O14793 Growth/differentiation factor 8) and 6 aa Histidine-based tag. It as a predicted molecular mass of 26.8 kDa (13.4 kDa under reducing conditions in SDSPAGE). Purity >97% by SDS-PAGE gel |
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| Product Specific References | - Rebbapragada, A. et al., 2003. Myostatin signals through a transforming growth factor beta-like signalling pathway to block adipogenesis. Mol Cell Biol., 23: 7230-42. - Matsakas, A. et al., 2009. Molecular, cellular and physiological investigation of myostatin propeptide-mediated muscle growth in adult mice. Neuromuscul. Disord., 19: 489-499. - Guo, T. et al., 2009. Myostatin inhibition in muscle, but not adipose tissue, decreases fat mass and improves insulin sensitivity. PLoS ONE, 4: e4937. - Sharma, M. et al., 1999. Myostatin, a transforming growth factor-beta superfamily member, is expressed in heart muscle and is upregulated in cardiomyocytes after infart. J. Cell Physiol., Jul, 180 (1): 1-9. |
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