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產(chǎn)品名稱

Mouse/Rat beta-Amyloid (1 - 40) ELISA Kit from EAGLE BIOSCIENCES INC.

產(chǎn)品貨號(hào)

ARG80980

產(chǎn)品價(jià)格

現(xiàn)貨詢價(jià)，電話：010-67529703

產(chǎn)品規(guī)格

1 x 96 well

產(chǎn)品品牌

EAGLE BIOSCIENCES INC.

產(chǎn)品概述

產(chǎn)品詳情

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Product Name	Mouse/Rat beta-Amyloid (1 - 40) ELISA Kit
Description	This ELISA kit is capable for measuring mouse and rat beta-Amyloid (1 - 40), it does not detect p3 peptide, which is cleaved by alpha or gamma secretase. This product is also useful for measuring mouse and rat derived beta-Amyloid in brain extract, cell culture supernatant and blood sample (serum or plasma) of mouse or rat. This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for beta-Amyloid (35 - 40) has been pre-coated onto a microtiter plate. Standards or samples are pipetted into the wells, and any beta-Amyloid (1 - 40) present is bound by the immobilized antibody. After washing away any unbound substances, a HRP-conjugated antibody specific for mouse/rat beta-Amyloid (1 - 16) is added to each well and incubate. After incubation, the capture antibody-antigen complex is developed and immobilized to the pre-coated wells. After washing away any unbound substances, a substrate solution (TMB) is added to the wells and color develops in proportion to the amount of beta-Amyloid (1 - 40) bound in the initial step. The color development is stopped by the addition of acid and the intensity of the color is measured at a wavelength of 450nm. The concentration of beta-Amyloid (1 - 40) in the sample is then determined by comparing the O.D of samples to the standard curve. The concentration of antigen is directly proportional to the optical density measured in the wells. Sample Types: brain extract, serum, plasma, cell culture supernatants. Range: 1.56-100 pg/ml. Sensitivity: 0.28 pg/ml. Time: 3 hours ? The first case of Alzheimer's disease was defined and reported in 1907 by the German scientist, Dr. A. Alzheimer. His studies have shown that this is the main cause of dementia in the elderly. The plaques which appear in the brains of Alzheimer's disease patients are mostly constituted by the Amyloid beta protein (A-beta). A-beta is a peptide which consists of 40 or 42 (43) amino acids, and reports show that this is cleaved by beta- and gamma- secretase from the amyloid precursor protein. In addition, the presence of numerous variant A-beta molecules has been demonstrated in the culture fluid of mouse neuroblastoma cells transfected with cDNA coding human amyloid precursor protein (APP). Furthermore, in 1995, a dominant and differential deposition of distinct beta amyloid peptide species, A-beta (N3pE), in senile plaques was found by Saido et al. This modified molecule, starting at the 3rd amino terminal residue, glutamate, was discovered to convert to pyroglutamate through intramolecular dehydration.
Size	1 x 96 well
Concentration	n/a
Applications	RUO
Other Names	beta-Amyloid (1 - 40)
Gene, Accession, CAS #	n/a
Catalog #	ARG80980
Price
Order / More Info	Mouse/Rat beta-Amyloid (1 - 40) ELISA Kit from EAGLE BIOSCIENCES INC.
Product Specific References	n/a
產(chǎn)品資料

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