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產(chǎn)品名稱

ANTI BETA2 GLYCOPROTEIN 1 IgM ELISA from EAGLE BIOSCIENCES INC.

產(chǎn)品貨號

DCM111

產(chǎn)品價格

現(xiàn)貨詢價，電話：010-67529703

產(chǎn)品規(guī)格

1 x 96 well

產(chǎn)品品牌

EAGLE BIOSCIENCES INC.

產(chǎn)品概述

產(chǎn)品詳情

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Product Name	ANTI BETA2 GLYCOPROTEIN 1 IgM ELISA
Description	Anti Beta 2 Glycoprotein 1 IgM ELISA Assay Kit is based on the initial binding of the antibodies present in calibrators, controls or pre-diluted samples to the beta 2 Glycoprotein 1 coated on the inner surface of the microplate wells. After a 60 minutes incubation the microplate is washed with a wash buffer to remove the non-reactive serum components. Then an anti-human-IgM horseradish peroxidase conjugated solution recognizes IgM class antibodies bound to the immobilized Antigenss. After a 30 minutes incubation the excess of enzyme conjugate, which is not specifically bound, is washed away with the wash buffer. Finally, a chromogenic substrate solution containing TMB is dispensed into the wells. After 15 minutes of incubation color development is stopped by adding the stop solution. The solution turns yellow at this point. The level of color is directly proportional to the concentration of IgM antibodies present in the original sample. The concentration of IgM antibodies present in the sample is calculated through a calibration curve. Sample Types: serum, plasma. Range: 10 - 160AU/mL. Sensitivity: 0.11 AU/mL. Time: 2 hours ? The antiphospholipid syndrome (APS) is a disorder that presents peculiar symptoms: arterial and venous thrombosis, thrombocytopenia, ulcers of the lower limbs, hemolytic anemia, loss of the fetus during pregnancy and is associated with the presence of antiphospholipid antibodies. Antiphospholipid antibodies represent a large and heterogeneous immunoglobulins group, including anticardiolipin antibodies and lupus anticoagulant. The former are diagnosed by on their reactivity with cardiolipin or other anionic phospholipids in ELISA test, while the latter is detected in phospholipid-dependent coagulation tests (aPTT, KCT, dRVVT). In early '90s it was observed that antiphospholipid antibodies are not directed against anionic phospholipids, as long it was considered, but they react with plasma proteins bound to anionic (phospholipidic) surfaces. In fact about cardiolipin, it was observed the need of a cofactor for antibodies binding, this cofactor was identified in β2 glycoprotein 1 (β2-GP1). The β2 glycoprotein 1 is a plasma glycoprotein of molecular weight of 50 kD, which is complexed to lipoproteins for 40%. A patient with clinical suspicion of APS, who has an high titre of anti-beta2GP1 antibodies, even if LAC or anti-cardiolipin antibodies are negative, has a strong chance of having the antiphospholipid syndrome, because these antibodies recognize β2-GP1 bound to the surface of phospholipid (cardiolipin). These proteins seem to express their Antigensicity only after contact with specific areas such as the anionic phospholipid surface or very hydrophilic plastic surfaces. The importance of ELISA anti-phospholipids test, including the anti-β2-GP1 test, lies in the fact that a positive test if associated with one or more of the symptoms can confirm a diagnosis of APS.
Size	1 x 96 well
Concentration	n/a
Applications	RUO
Other Names	ANTI-BETA2 GLYCOPROTEIN, Beta, 2, anti, IgM
Gene, Accession, CAS #	n/a
Catalog #	DCM111
Price
Order / More Info	ANTI BETA2 GLYCOPROTEIN 1 IgM ELISA from EAGLE BIOSCIENCES INC.
Product Specific References	n/a
產(chǎn)品資料

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