| 產(chǎn)品詳情 |
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| Product Name | Human Neuroserpin ELISA Kit |
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| Description | This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for Neuroserpin has been pre-coated onto a microtiter plate. Standards or samples are pipetted into the wells and any Neuroserpin present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-conjugated antibody specific for Neuroserpin is added to each well and incubate. Following a washing to remove unbound substances, streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After washing away any unbound antibody-enzyme reagent, a substrate solution (TMB) is added to the wells and color develops in proportion to the amount of Neuroserpin bound in the initial step. The color development is stopped by the addition of acid and the intensity of the color is measured at a wavelength of 450nm ±2nm.The concentration of Neuroserpin in the sample is then determined by comparing the O.D of samples to the standard curve. Sample Types: serum, plasma, cell culture supernatants. Range: 62.5-4000 pg/ml. Sensitivity: 30 pg/mL. Time: 4 hours ? Neuroserpin is an inhibitory serpin that is expressed predominantly in central nervous system. Although the physiological target of neuroserpin is still unclear, cumulative evidence suggest that it plays an important role in controlling proteolytic degradation of extracellular matrix (ECM) during synaptogenesis and the subsequent development of neuronal plasticity. In the adult brain, neuroserpin is secreted from the growth cones of neurons in areas where synaptic changes are associated with learning and memory, i.e. cerebral cortex, hippocampus, and amygdala. The neuroprotective role of neuroserpin has been demonstrated in transgenic mice lacking neuroserpin expression. The deficiency of neuroserpin in these mice was associated with motor neuron disease characterized by axonal degradation. In humans, defects in neuroserpin, caused by point mutations in the neuroserpin gene, underlie a hereditary disorder called the familial encephalopathy with neuroserpin inclusion bodies (FENIB). Recombinant human neuroserpin is a 44.6 kDa non-glycosylated protein containing 394 amino-acid residues. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | Neuroserpin |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | ARG80186 |
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| Price | |
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| Order / More Info | Human Neuroserpin ELISA Kit from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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