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產(chǎn)品名稱

High Sensitive Human Fetuin-A ELISA Assay Kit from EAGLE BIOSCIENCES INC.

產(chǎn)品貨號

HFA31-K01

產(chǎn)品價格

現(xiàn)貨詢價，電話：010-67529703

產(chǎn)品規(guī)格

1 x 96 well

產(chǎn)品品牌

EAGLE BIOSCIENCES INC.

產(chǎn)品概述

產(chǎn)品詳情

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Product Name	High Sensitive Human Fetuin-A ELISA Assay Kit
Description	This Eagle Biosciences High Sensitive Human Fetuin-A ELISA Assay Kit is designed, developed and produced for the quantitative measurement of human Fetuin-A in serum samples. The assay utilizes the two-site ``sandwich'' technique with two selected goat anti-human Fetuin-A polyclonal antibodies that bind to different epitopes of human Fetuin-A. Assay standards, controls and prediluted patient serum samples containing human Fetuin-A are added to microtiter wells of a microplate that was coated with a high affinity polyclonal goat anti-human Fetuin-A antibody. After the first incubation period, the antibody on the wall of microtiter well captures human Fetuin-A in the sample and unbound proteins in each microtiter well are washed away. Then a horseradish peroxidase (HRP)-conjugated polyclonal anti-human Fetuin-A antibody is added to each microtiter well and a ``sandwich'' of ``capture antibody - human Fetuin-A - HRP-conjugated tracer antibody'' is formed. The unbound tracer antibody is removed in the subsequent washing step. HRP-conjugated tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to the Fetuin-A on the wall of the microtiter well is directly proportional to the amount of Fetuin-A in the sample. A standard curve is generated by plotting the absorbance versus the respective human Fetuin-A concentration for each standard on point-to-point or cubical scales. The concentration of human Fetuin-A in test samples is determined directly from this standard curve. Sample Types: serum. Range: 0 -4343 ng/mL. Sensitivity: 40 ng/mL. Time: 3 hours ? Fetuin-A, also known as alpha-2-HS glycoprotein, is a 59 kDa glycoprotein that consists of two amino-terminal cystatin domains and a smaller carboxyl-terminal domain. Fetuin-A is synthesized by the liver and secreted into blood stream, where its concentration in adult mammals ranges from 0.5 - 1.5 g/L. Fetuin-A occurs in high serum concentration during fetal life and involves in protease inhibitory activities and development-associated regulation of calcium metabolism and osteogenesis. It accumulates in bones and teeth as a major fraction of noncollagenous bone proteins. Biologically, studies have demonstrated that Fetuin-A is the major calcification inhibitor found in serum, where it interferes with calcium salt precipitation. A recent study has indicated that Fetuin-A level drops in uremic patients on hemodialysis in comparison to normal healthy controls. The low Fetuin-A level may be associated with a higher cardiovascular mortality in patients on dialysis.
Size	1 x 96 well
Concentration	n/a
Applications	RUO
Other Names	Human Fetuin-A, sensitive
Gene, Accession, CAS #	n/a
Catalog #	HFA31-K01
Price
Order / More Info	High Sensitive Human Fetuin-A ELISA Assay Kit from EAGLE BIOSCIENCES INC.
Product Specific References	n/a
產(chǎn)品資料

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