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產(chǎn)品名稱

Cell Viability Assay Kit (green fluorescence) from AAT BIOQUEST, INC.

產(chǎn)品貨號(hào)

22786

產(chǎn)品價(jià)格

現(xiàn)貨詢價(jià)，電話：010-67529703

產(chǎn)品規(guī)格

500 tests

產(chǎn)品品牌

AAT BIOQUEST, INC.

產(chǎn)品概述

產(chǎn)品詳情

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Product Name	Cell Viability Assay Kit (green fluorescence)
Description	Cell Meter? Cell Viability Assay Kit Green Fluorescence. Our Cell Meter? assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This kit uses the non-fluorescent calcein AM that becomes strongly fluorescent upon entering into live cells. Calcein AM is a hydrophobic compound that easily permeates intact live cells. The hydrolysis of the non-fluorescent calcein AM by intracellular esterases generates the strongly fluorescent hydrophilic calcein that is well-retained in the cell cytoplasm. The esterase activity is proportional to the number of viable cells, and thus directly related to the fluorescence intensity of calcein generated from the esterase-catalyzed hydrolysis of calcein AM. Cells grown in black-walled plates can be stained and quantified in less than two hours. The assay is more robust than the tetrazolium salt or Alarmar Blue?-based assays. It can be readily adapted for high-throughput assays in a wide variety of fluorescence platforms such as microplate assays, immunocytochemistry and flow cytometry. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components with an optimized cell-labeling protocol. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells. Using 100 ul of reagents per well in a 96-well format, this kit provides sufficient reagents to perform 500 assays. Using 25 ul of reagents per well in a 384-well format, this kit provides sufficient reagents to perform 2000 assays.
Size	500 tests
Concentration	n/a
Applications	Biossay, Fluorometric
Other Names	n/a
Gene, Accession, CAS #	n/a
Catalog #	22786
Price
Order / More Info	Cell Viability Assay Kit (green fluorescence) from AAT BIOQUEST, INC.
Product Specific References	n/a
產(chǎn)品資料

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