| Description | Prostaglandin F2alpha (PGF2alpha) is derived from PGH2 which in turn is synthesized from arachidonic acid through the cyclooxygenase pathway. It is produced in various tissues. It contracts smooth muscle and has been used to induce labor. It has a luteolytic effect and has been considered a luteolytic hormone. Kit Components: P9053-35E EIA Buffer: 1x30ml. To be used to dilute enzyme conjugate and PGF2a standards. P9053-35EF Wash Buffer (10X): 1x20ml. To be diluted 10x with deionized water. This is used to wash all unbound enzyme conjugate, samples and standards from the plate after the one hour incubation. P9053-35G Substrate: 1x20ml. Stabilized 3,3', 5,5' Tetramethylbenzidine (TMB) plus Hydrogen Peroxide (H2O2) in a single bottle. It is used to develop the color in the wells after they have been washed. P9053-35H Extraction Buffer (5X): 1x30ml. To be diluted 5x with deionized water. This is used for diluting extracted and non-extracted samples. P9053-35J Prostaglandin F2a (HRP): 1x150 |
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