| Description | Purity: > 90% by SDS - PAGE Format: Liquid. In 20mM Tris-Hcl buffer (pH8.5) containing 1mM DTT,0.15M NaCl, 01mM PMSF, 1mM EDTA, 30% glycerol.. IL34, also known as interleukin-34 isoform 1, is a secreted cytokine. This protein is expressed in a variety of tissues richest in the spleen and stimulates the proliferation of monocytes. In a functional study, IL-34, such as CSF-1, another ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1 / 2) in human monocytes. In addition, it promoted the formation of macrophage precursor colonization units-macrophages (CFU-M) in human bone marrow cultures. Recombinant human IL34, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
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