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產(chǎn)品名稱

Rat Glicentin ELISA Assay Kit from EAGLE BIOSCIENCES INC.

產(chǎn)品貨號

YK111

產(chǎn)品價格

現(xiàn)貨詢價，電話：010-67529703

產(chǎn)品規(guī)格

1 x 96 well

產(chǎn)品品牌

EAGLE BIOSCIENCES INC.

產(chǎn)品概述

產(chǎn)品詳情

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Product Name	Rat Glicentin ELISA Assay Kit
Description	This Rat Glicentin ELISA Assay kit for determination of rat glicentin in plasma is based on a competitive enzyme immunoassay using combination with highly specific antibody to rat glicentin and biotin - avidin affinity system. The 96 wells plate is coated with goat anti rabbit IgG and rat glicentin standard or samples, biotinylated rat glicentin and rabbit anti rat glicentin antibody are added to the wells for competitive immunoreaction. After incubation and plate washing, HRP labeled streptoavidins are added to bind to the Antigens-antibody complex so that HRP labeled streptoavidin - biotinylated rat glicentin - antibody complexs are formed on the surface on the wells. Finally, excess HRP labeled streptoavidins are rinsed out and HRP enzyme activity is determined and the concentration of rat glicentin is calculated. Sample Types: rat plasma. Range: 2.06 - 50 pmol/mL. Time: 20 hours ? Glicentin is a 69-amino-acid peptide containing glucagon and oxyntomodulin sequences in the molecule. It is suggested that glicentin and oxyntomodulin are produced in the intestinal L-cells and glucagon in A-cells in the pancreas, these peptides are derived from a common precursor by two different tissue-specific processing pathways. In 1983, the amino acid sequence of human glicentin was deduced by Bell et al. from the genomic sequence of human preproglucagon. Glicentin is a major form of gut glucagon-like immunoreactants (Gut GLIs). In mammalian small intestine, proglucagon is processed into glicentin, oxyntomodulin, and glucagon -like peptide (GLP)-1 and -2. GLP-1(7-37) and GLP-1(7-36) amide have been isolated from the intestine and pancreas. It has been known that the GLP-1 sequence is well conserved between species in all mammals studied. Using synthetic peptides, several investigators have demonstrated that in contrast to GLP-1(1-37), truncated GLP-1(7-36) amide and GLP-1(7-37) have several physiological effects. However, the physiological role of glicentin, a major gut glucagon, is still unclear. It has been known that the circulating level of plasma glicentin-like peptides increases significantly nutrient ingestion.
Size	1 x 96 well
Concentration	n/a
Applications	RUO
Other Names	Rat Glicentin ELISA Assay Kit, YK111, sceti
Gene, Accession, CAS #	n/a
Catalog #	YK111
Price
Order / More Info	Rat Glicentin ELISA Assay Kit from EAGLE BIOSCIENCES INC.
Product Specific References	n/a
產(chǎn)品資料

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