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產(chǎn)品名稱

A549 Whole Cell Lysate from ROCKLAND IMMUNOCHEMICALS INC.

產(chǎn)品貨號

W09-001-372

產(chǎn)品價(jià)格

現(xiàn)貨詢價(jià)，電話：010-67529703

產(chǎn)品規(guī)格

500 μg

產(chǎn)品品牌

ROCKLAND IMMUNOCHEMICALS INC.

產(chǎn)品概述

產(chǎn)品詳情

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Product Name	A549 Whole Cell Lysate
Description	The cells were grown in Dulbecco's medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to lyse the cells. Protein integrity was ensured using a cocktail of protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases (0.1 mM AEBSF HCl, 0.08 μM Aprotinin, 5 μM Bestatin, 1.5 μM E-64, 2 μM Leupeptin Hemisulfate, 1 μM Pepstatin A). Phosphatase inhibitors 1 mM NaF and 1 mM Na3VO4 were also added. Cell debris was removed by centrifugation. Protein concentration was determined by a modified Lowry assay using a commercially available kit. Protein concentration was adjusted to 2 mg/ml and then an equal volume of 2X SDS-PAGE sample buffer was added.
Size	500 μg
Concentration	1.0 mg/mL
Applications	Western Blot. Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95° C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior to heating. The recommended loading volume per lane is 10-20 μl depending on the size format of your gel.
Other Names	A549 Whole Cell Lysate, A549 WCL, A549 Lysate
Gene, Accession, CAS #	n/a
Catalog #	W09-001-372
Price
Order / More Info	A549 Whole Cell Lysate from ROCKLAND IMMUNOCHEMICALS INC.
Product Specific References	n/a
產(chǎn)品資料

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