| Description | The Human SCGN / Secretagogin ELISA Pair Set is for the quantitative determination of Human SCGN / Secretagogin.This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for SCGN / Secretagogin coated on a 96-well plate. Standards and samples are added to the wells, and any SCGN / Secretagogin present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-SCGN / Secretagogin monoclonal antibody is then added, producing an antibody-antigen-antibody sandwich. The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of SCGN / Secretagogin present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm. |
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