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產(chǎn)品名稱

Fluo-8 Medium Removal Calcium Assay Kit (for difficult cell lines) from AAT BIOQUEST, INC.

產(chǎn)品貨號

36309

產(chǎn)品價格

現(xiàn)貨詢價，電話：010-67529703

產(chǎn)品規(guī)格

100 plates

產(chǎn)品品牌

AAT BIOQUEST, INC.

產(chǎn)品概述

產(chǎn)品詳情

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Product Name	Fluo-8 Medium Removal Calcium Assay Kit (for difficult cell lines)
Description	Screen Quest? Fluo-8 Medium Removal Calcium Assay Kit Optimized for Difficult Cell Lines. Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR). Screen Quest? Fluo-8 NW Calcium Assay Kit provides a homogeneous fluorescence-based assay for detecting the intracellular calcium mobilization. Cells expressing a GPCR of interest that signals through calcium are pre-loaded with our proprietary Fluo-8 NW which can cross cell membrane. Fluo-8 NW is the brightest calcium indicator available for HTS screening. Once inside the cell, the lipophilic blocking groups of Fluo-8 NW are cleaved by non-specific cell esterase, resulting in a negatively charged fluorescent dye that stays inside cells, and its fluorescence is greatly enhanced upon binding to calcium. When cells stimulated with screening compounds, the receptor signals release of intracellular calcium, which greatly increase the fluorescence of Fluo-8 NW. The characteristics of its long wavelength, high sensitivity, and 100-250 times fluorescence increases (when it forms complexes with calcium) make Fluo-8 NW an ideal indicator for measurement of cellular calcium. This Screen Quest Fluo-8 NW Calcium Assay Kit provides an optimized assay method for monitoring G-protein-coupled receptors (GPCRs) and calcium channels. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation.
Size	100 plates
Concentration	n/a
Applications	Biossay
Other Names	n/a
Gene, Accession, CAS #	n/a
Catalog #	36309
Price
Order / More Info	Fluo-8 Medium Removal Calcium Assay Kit (for difficult cell lines) from AAT BIOQUEST, INC.
Product Specific References	n/a
產(chǎn)品資料

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