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產(chǎn)品名稱

1,25-Dihydroxy Vitamin D EIA from IMMUNODIAGNOSTIC SYSTEMS

產(chǎn)品貨號

AC-62F1

產(chǎn)品價格

現(xiàn)貨詢價，電話：010-67529703

產(chǎn)品規(guī)格

96 wells

產(chǎn)品品牌

IMMUNODIAGNOSTIC SYSTEMS

產(chǎn)品概述

產(chǎn)品詳情

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Product Name	1,25-Dihydroxy Vitamin D EIA
Description	The IDS 1,25-Dihydroxy Vitamin D EIA kit is a complete assay system for the purification of 1,25D in patient samples by immunoextraction followed by quantitation by EIA. Patient samples are delipidated and 1,25D extracted from potential cross-reactants by incubation for 90 minutes with a highly specific solid phase monoclonal anti-1,25D. The immunoextraction gel is then washed and purified 1,25D eluted directly into glass assay tubes. Reconstituted eluates and calibrators are incubated overnight with a highly specific sheep anti-1,25D. Then a portion of this is incubated for 90 minutes with shaking in microplate wells which are coated with a specific anti-sheep antibody. 1,25D linked to biotin is then added and the plate shaken for a further 60 minutes before aspiration and washing. Enzyme (horseradish peroxidase) labelled avidin is added and binds selectively to complexed biotin and, following a further wash step, colour is developed using a chromogenic substrate (TMB). The absorbance of the stopped reaction mixtures are read in a microtitre plate reader, colour intensity developed being inversely proportional to the concentration of 1,25D.
Size	96 wells
Concentration	n/a
Applications	colorimetric ELISA or EIA
Other Names	1,25-dihydroxyvitamin D; 1,25D; 1,25OH D kit, assay, immunoassay
Gene, Accession, CAS #	n/a
Catalog #	AC-62F1
Price
Order / More Info	1,25-Dihydroxy Vitamin D EIA from IMMUNODIAGNOSTIC SYSTEMS
Product Specific References	n/a
產(chǎn)品資料

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