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產(chǎn)品名稱

Brucella IgM ELISA Kit from DRG INTERNATIONAL INC.

產(chǎn)品貨號

EIA3456R

產(chǎn)品價(jià)格

現(xiàn)貨詢價(jià)，電話：010-67529703

產(chǎn)品規(guī)格

96 wells

產(chǎn)品品牌

DRG INTERNATIONAL INC.

產(chǎn)品概述

產(chǎn)品詳情

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Product Name	Brucella IgM ELISA Kit
Description	An enzyme immunoassay for the qualitative and semiquantitative determination of IgM-class antibodies to Brucella in serum and plasma. The DRG Brucella IgM ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) Patient samples are diluted with Sample Diluent and additionally incubated with IgG-RF-Sorbent, containing hyper-immune anti-human IgG-class antibody to eliminate competitive inhibition from specific IgG and to remove rheumatoid factors. This pretreatment avoids false negative or false positive results. Microtiter wells as a solid phase are coated with inactivated Brucella abortus Antigen from strain S99-Weybridge. Diluted patient specimens and ready-for-use controls are pipetted into these wells. During incubation Brucella-specific antibodies of positive specimens and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgM antibodies are dispensed into the wells. During a second incubation this anti-IgM conjugate binds specifically to IgM antibodies resulting in the formation of enzyme-linked immune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Brucella-specific IgM antibody in the patient specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.
Size	96 wells
Concentration	n/a
Applications	n/a
Other Names	EIA, enzyme immunoassay, assay
Gene, Accession, CAS #	n/a
Catalog #	EIA3456R
Price
Order / More Info	Brucella IgM ELISA Kit from DRG INTERNATIONAL INC.
Product Specific References	n/a
產(chǎn)品資料

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