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產(chǎn)品名稱

RatLaps?(CTX-I) EIA from IMMUNODIAGNOSTIC SYSTEMS

產(chǎn)品貨號(hào)

AC-06F1

產(chǎn)品價(jià)格

現(xiàn)貨詢價(jià)，電話：010-67529703

產(chǎn)品規(guī)格

96 wells

產(chǎn)品品牌

IMMUNODIAGNOSTIC SYSTEMS

產(chǎn)品概述

產(chǎn)品詳情

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Product Name	RatLaps?(CTX-I) EIA
Description	The RatLaps? (CTX-I) is based upon the competitive binding of a polyclonal antibody to soluble RatLaps antigens EKSQDGGR or to immobilized RatLaps antigens. Briefly, the polyclonal antibody is raised against a synthetic peptide having a sequence (EKSQDGGR) specific for a part of the C-terminal telopeptide α1 chain of rat type I collagen. For standardization of the RatLaps? (CTX-I) a synthetic peptide (EKSQDGGR), which is specific for the C-terminal telopeptide α1 chain of type I collagen in rats has been used. During the pre-incubation step, biotinylated EKSQDGGR is immobilized by binding to the streptavidin-coated microtitre wells. The wells are emptied and washed. Standards, control, or unknown samples (culture supernatant or rat/mouse serum or urine) are pipette into appropriate wells, followed by a solution of a primary antibody (polyclonal rabbit). Following the primary-incubation step the wells are emptied and washed. In the secondary-incubation step a solution of a Goat anti-Rabbit antibody conjugated with peroxidase (secondary antibody) is added and binds to the polyclonal rabbit antibody. After the third washing step a chromogenic substrate (TMB) is added and the color reaction is stopped with sulfuric acid. Finally, the absorbence at 450 nm is measured with 650 nm as reference if possible. The absorbence level is inversely related to concentration of RatLaps antigens in the sample.
Size	96 wells
Concentration	n/a
Applications	colorimetric ELISA or EIA
Other Names	fragments of type I collagen kit, assay, immunoassay
Gene, Accession, CAS #	n/a
Catalog #	AC-06F1
Price
Order / More Info	RatLaps™(CTX-I) EIA from IMMUNODIAGNOSTIC SYSTEMS
Product Specific References	n/a
產(chǎn)品資料

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