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產(chǎn)品名稱

Cystatin C EIA Kit from STRESSMARQ BIOSCIENCES INC.

產(chǎn)品貨號

SKT-219-96

產(chǎn)品價格

現(xiàn)貨詢價，電話：010-67529703

產(chǎn)品規(guī)格

96 well

產(chǎn)品品牌

STRESSMARQ BIOSCIENCES INC.

產(chǎn)品概述

產(chǎn)品詳情

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Product Name	Cystatin C EIA Kit
Description	The Cystatin C EIA kit is designed to quantitatively measure human Cystatin C present in biological samples and tissue culture media. A human Cystatin C standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with a monoclonal antibody to capture the Cystatin C present. After a 60 minute incubation, the plate is washed and a peroxidase conjugated Cystatin C monoclonal antibody is added. The plate is again incubated for 30 minutes and washed. Substrate is then added to the plate, which reacts with the bound Cystatin C Antibody Conjugate. After a third incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the Cystatin C in the sample is calculated, after making suitable correction for dilution, using software available with most plate readers. Sandwich EIA (Enzyme Immunoassay). Species Reactivity: human. Intra Assay Precision: Four human samples were diluted with Assay Buffer and run in replicates of 20 in an assay. The mean and precision of the calculated Cystatin C concentrations were: Sample 1- 7.72 ng/mL, 9.1% CV Sample 2- 5.08 ng/mL, 10.3% CV Sample 3- 4.42 ng/mL, 7.6% CV Sample 4- 0.88 ng/mL, 10.3% CV Inter Assay Precision: Four human samples were diluted with Assay Buffer and run in duplicates in ten assays run over multiple days by three operators. The mean and precision of the calculated Cystatin C concentrations were: Sample 1- 7.79 ng/mL, 8.4% CV Sample 2- 5.48 ng/mL, 10.2% CV Sample 3- 4.97 ng/mL, 11.1% CV Sample 4- 1.04 ng/mL, 12.40% CV. Range: 0.156 - 10 ng/ml. Sensitivity: 0.058 ng/ml. Time: 2 hours.
Size	96 well
Concentration	n/a
Applications	EIA kit used to measure cystatin C levels in samples. RUO
Other Names	StressXpress? Cystatin 3 EIA Kit, gamma trace EIA Kit, post-gamma-globulin or neuroendocrine basic polypeptide EIA Kit
Gene, Accession, CAS #	n/a
Catalog #	SKT-219-96
Price
Order / More Info	Cystatin C EIA Kit from STRESSMARQ BIOSCIENCES INC.
Product Specific References	n/a
產(chǎn)品資料

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