| Description | Rat IgG1 kappa Isotype Control (eBRG1), eBioscience?. The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel |
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| Product Specific References | PubMed: 19179415, 19490961, 21147109, 21727190, 21918185, 22253596, 22642909, 22723864, 23181795, 24222381, 24670801, 24778275, 24958709, 24980747, 25121619, 25249168, 25472994, 26056255, 26451303, 27044016, 28346226 |
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