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產(chǎn)品名稱(chēng)

Mouse MIP-2 ELISA KIT from EAGLE BIOSCIENCES INC.

產(chǎn)品貨號(hào)

MIP11-K01

產(chǎn)品價(jià)格

現(xiàn)貨詢(xún)價(jià)，電話(huà)：010-67529703

產(chǎn)品規(guī)格

1 x 96 well

產(chǎn)品品牌

EAGLE BIOSCIENCES INC.

產(chǎn)品概述

產(chǎn)品詳情

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Product Name	Mouse MIP-2 ELISA KIT
Description	The Eagle Biosciences Mouse Macrophage Inflammatory Protein 2 (MIP-2) ELISA Assay Kit employs quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for MIP-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MIP-2 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for MIP-2 is added to the wells and binds to the combination of capture antibody-MIP-2 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of MIP-2 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven MIP-2 standard dilutions and MIP-2 sample concentration determined. Sample Types: serum, plasma, cell culture. Range: 7.8 - 250 pg/ml. Sensitivity: 4 pg/ml. Time: 3.0 hours ? Mouse macrophage inflammatory protein-2 (MIP-2), also known as CXCL2, was originally identified as a heparin-binding protein secreted by an LPS-stimulated mouse macrophage cell line (1). A cDNA clone encoding the protein was isolated from this cell line and characterized (2). Based on its protein and DNA sequences, mouse MIP-2 was classified as a member of the alpha (CXC) chemokine family of inflammatory and immunoregulatory cytokines (3). Mouse MIP-2 cDNA encodes a 100 amino acid residue precursor protein from which the amino-terminal 27 amino acid residues are cleaved to generate the mature mouse MIP-2. The protein sequence of mouse MIP-2 shows approximately 63% identity to that of mouse KC, another mouse alpha chemokine. Mouse MIP-2 is also 60% identical to human GROβ and GROγ(2). Based on these protein sequence similarities, it is likely that mouse KC and MIP-2 are homologs of human GROα, β and γ chemokines. Since chemokines with protein sequence homology to human IL-8 have not been identified in mice, it has been suggested that the mouse KC and MIP-2 are functional homologs of human IL-8 in mice (3, 4). A putative mouse homolog of the human IL-8 receptor beta (IL-8 Rβ) has also been cloned. This receptor shows 71% identity to human IL-8 Rβ and 68% identity to human IL-8 Rα. Both mouse KC and MIP-2 bind mouse IL-8 Rβ with high affinity (5). Like human IL-8, mouse MIP-2 exhibits potent neutrophil chemotactic activity and may be a key mediator of neutrophil recruitment in response to tissue injury and infection (3, 4). Increased MIP-2 expression has been found to be associated with neutrophil influx in various inflammatory conditions (6 - 10).
Size	1 x 96 well
Concentration	n/a
Applications	RUO
Other Names	MIP-2,Macrophage inflammatory protein-2
Gene, Accession, CAS #	n/a
Catalog #	MIP11-K01
Price
Order / More Info	Mouse MIP-2 ELISA KIT from EAGLE BIOSCIENCES INC.
Product Specific References	n/a
產(chǎn)品資料

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