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產(chǎn)品名稱

LEPTIN-Sensitive-ELISA from EAGLE BIOSCIENCES INC.

產(chǎn)品貨號

E077

產(chǎn)品價格

現(xiàn)貨詢價，電話：010-67529703

產(chǎn)品規(guī)格

1 x 96 well

產(chǎn)品品牌

EAGLE BIOSCIENCES INC.

產(chǎn)品概述

產(chǎn)品詳情

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Product Name	LEPTIN-Sensitive-ELISA
Description	The Eagle Biosciences Leptin Sensitive ELISA is a so-called Sandwich-Assay using two specific and high affinity antibodies. The Leptin in the samples binds to the first antibody coated on the microtiter plate. In the following step the second specific anti-Leptin-Antibody binds in turn to the immobilised Leptin. The second antibody is biotinylated and will be applied in a mixture with a Streptavidin-Peroxidase-Enzyme Conjugate. In the closing substrate reaction the turn of the colour will be catalysed quantitatively depending on the Leptin-level of the samples. Sample Types: serum, plasma, urine, saliva, cell culture. Range: 0.05 - 5 ng/ml. Sensitivity: 0.01 ng/ml. Time: 1.75 hours ? Leptin, the product of the ob gene (1,2), is a recently discovered single-chain proteohormone with a molecular weight of 16 kD, which is thought to play a key role in the regulation of body weight. Its amino acid sequence exhibits no major homologies with other proteins (1). Leptin is almost exclusively produced by differentiated adipocytes (3-5). It acts on the central nervous system, in particular the hypothalamus, thereby suppressing food intake and stimulating energy expenditure (2,6-9). Leptin receptors - alternatively spliced forms exist that differ in length - belong to the cytokine class I receptor family (10-12). They are found ubiquitously in the body (10, 11, 13, 14) indicating a general role of leptin, which is currently not fully understood. A circulating form of the leptin receptor exists which acts as one of several leptin binding proteins (15). Besides its metabolic effects, leptin was shown to have a strong influence on a number of Endocrinology axes. In male mice, it blunted the starvation-induced marked decline of LH, testosterone, thyroxine and the increase of ACTH and corticosterone. In female mice, leptin prevented the starvation-induced delay in ovulation (16). Ob/ob mice, which are leptin deficient due to an ob gene mutation, are infertile. This defect could be corrected by administration of leptin, but not through weight loss due to fasting (17), suggesting that leptin is pivotal for reproductive functions.
Size	1 x 96 well
Concentration	n/a
Applications	RUO
Other Names	Leptin
Gene, Accession, CAS #	n/a
Catalog #	E077
Price
Order / More Info	LEPTIN-Sensitive-ELISA from EAGLE BIOSCIENCES INC.
Product Specific References	n/a
產(chǎn)品資料

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