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產(chǎn)品名稱

Mouse IGF-I, recombinant protein from IMMUNOLOGICAL & BIOCHEMICAL TESTSYSTEMS GmbH

產(chǎn)品貨號

rmIGF1-10

產(chǎn)品價格

現(xiàn)貨詢價，電話：010-67529703

產(chǎn)品規(guī)格

10 μg

產(chǎn)品品牌

IMMUNOLOGICAL & BIOCHEMICAL TESTSYSTEMS GmbH

產(chǎn)品概述

產(chǎn)品詳情

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Product Name	Mouse IGF-I, recombinant protein
Description	Product Specifications Mouse IGF-I: Recombinant Murine Insulin Like Growth Factor-I (IGF-1) Source: E.Coli Product Code: rmIGF-I Background: Murine IGF-I is a 70 amino acid peptide. Four of them differ from the human sequence, one is different from rat IGF-I. Description: Recombinant murine IGF-1 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7600 Dalton. IGF-I is purified by chromatographic techniques. Link to Sequence.Formulation: Sterile Filtered White lyophilized (freeze-dried) powder. Recombinant Murine IGF-1 was lyophilized after extensive dialysis against PBS. Solubility: It is recommended to reconstitute the lyophilized Murine IGF-I in sterile double distilled water not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability: Lyophilized Murine IGF-I although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution Murine IGF-1 should be stored at 4 C between 2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Pro-Glu-Thr-Leu. N-terminal methionine has been completely removed enzymatically. Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis. Biological Activity: Murine IGF-I is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation of murine BALB\\C 3T3 cells (measured by 3H-thymidine uptake) is less then 1.0 ng/ml, corresponding to a specific activity of 1x10 U/mg. Endotoxin: Less than 0.1 ng/μg (IEU/μg) of IGF-I.
Size	10 μg
Concentration	n/a
Applications	n/a
Other Names	Igf-1, insulin-like growth factor-1, Somatomedin c, ILGF1, Mechano growth factor
Gene, Accession, CAS #	n/a
Catalog #	rmIGF1-10
Price
Order / More Info	Mouse IGF-I, recombinant protein from IMMUNOLOGICAL & BIOCHEMICAL TESTSYSTEMS GmbH
Product Specific References	1. Fernandez et al.: Calcineurin in Reactive Astrocytes Plays a Key Role in the Interplay between Proinflammatory and Anti-Inflammatory Signals. The Journal of Neuroscience, August 15, 2007, 27(33):8745-8756. Link to Article.
產(chǎn)品資料

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