| Description | The Mouse IL-16 / Interleukin-16 ELISA Pair Set is for the quantitative determination of Mouse IL-16 / Interleukin-16.The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Mouse IL-16 / Interleukin-16 coated on a 96-well plate. Standards and samples are added to the wells, and any Mouse IL-16 / Interleukin-16 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Mouse IL-16 / Interleukin-16 monoclonal antibody is then added, producing an antibody-antigen-antibody sandwich. The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Mouse IL-16 / Interleukin-16 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm. |
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