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| Antigenic Specificity | Akt phospho S473 |
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| Clone | 17F6.B11 |
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| Host Species | Mouse |
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| Reactive Species | human, mouse, rat, monkey |
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| Isotype | IgG1 kappa |
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| Format | Protein A purified |
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| Size | 25 μL |
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| Concentration | 1.0 mg/mL |
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| Applications | ELISA, Flow Cytometry, Immunohistochemistry, IF Microscopy, Western Blot. This monoclonal antibody is suitable for ELISA, immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry use formalin-fixed paraffin-embedded sections. No pre-treatment of sample is required. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | Anti-AKT pS473 (MOUSE) Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human and mouse AKT protein phosphorylated at S473. A BLAST analysis was used to suggest cross-reactivity with AKT pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined. |
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| Immunogen | Anti-AKT pS473 antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein, followed by hybridoma development |
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| Other Names | mouse anti-AKT pS473 Antibody, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1 |
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| Gene, Accession # | AKT1, Gene ID: 207, NCBI: 62241011, UniProt: P31749 |
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| Catalog # | 200-301-268S |
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| Price | |
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| Order / More Info | Akt phospho S473 Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | n/a |
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