| 產(chǎn)品詳情 |
| Edit |   |
| Antigenic Specificity | Akt phospho S473, internal |
| Clone | 17F6.B11 |
| Host Species | Mouse |
| Reactive Species | human, mouse, rat, monkey |
| Isotype | IgG1 kappa |
| Format | Protein A purified |
| Size | 100 μg |
| Concentration | 1.0 mg/ml |
| Applications | ELISA, Flow Cytometry, Immunohistochemistry, IF Microscopy, Western Blot. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry use formalin-fixed paraffin-embedded sections. No pre-treatment of sample is required. |
| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
| Description | Anti-AKT pS473 Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein A chromatography. This phospho specific monoclonal antibody is specific for phosphorylated human and mouse AKT protein at S473. A BLAST analysis was used to suggest cross-reactivity with AKT pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined. |
| Immunogen | Anti-AKT pS473 (MOUSE) Monoclonal Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein, followed by hybridoma development. |
| Other Names | mouse anti-AKT pS473 Antibody, phospho AKT, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1 |
| Gene, Accession # | AKT1, Gene ID: 207, NCBI: 62241011, UniProt: P31749 |
| Catalog # | 200-301-268 |
| Price | |
| Order / More Info | Akt phospho S473, internal Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
| Product Specific References | n/a |
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