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產(chǎn)品名稱

Uridine diphospho-glucuronosyltransferases Activity Assay / Ligand Screening Kit from CREATIVE BIOMART

產(chǎn)品貨號

Kit-2109

產(chǎn)品價(jià)格

現(xiàn)貨詢價(jià)，電話：010-67529703

產(chǎn)品規(guī)格

100 assays

產(chǎn)品品牌

CREATIVE BIOMART

產(chǎn)品概述

產(chǎn)品詳情

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Product Name	Uridine diphospho-glucuronosyltransferases Activity Assay / Ligand Screening Kit
Description	UGT Activity Assay / Ligand Screening Kit enables rapid measurement of native or recombinant UGT activity in biological samples such as liver microsomes and can also be used to assess the effect of drugs and other novel compounds on UGT activity. The assay utilizes a highly fluorescent UGT substrate with a large Stokes shift (Ex/Em = 415/502 nm) that allows determination of UGT activity by tracking the drop in fluorescence emission as the substrate is converted into a non-fluorescent glucuronide. The multiisozyme substrate is glucuronidated by virtually all of the pharmacologically-relevant mammalian UGT1A and UGT2B enzymes. UGT specific activity is calculated by comparing the fluorescence loss versus a control reaction performed in the absence of the required cofactor UDPGA. The kit includes the pore-forming peptide antibiotic Alamethicin, which allows the UGT Substrate and UDPGA to rapidly diffuse across lipid membranes to access the UGT active site located in the lumen of microsomes. For verification of modulation of UGT activity by test ligands, diclofenac, a competitive inhibitor of most human and rodent UGT isozymes, is also included. The assay is highly sensitive, simple to perform and high-throughput adaptable. This assay can detect less than 0.1 mU UGT activity in biological samples. The kit contains a complete set of reagents sufficient for performing 100 reactions at a 100 mμL reaction volume.
Size	100 assays
Concentration	n/a
Applications	Rapid assessment of native/recombinant UGT activity in fractions prepared from tissues and cells.Screening of drugs and novel ligands for interaction with native/recombinant UGT enzymes.
Other Names	n/a
Gene, Accession, CAS #	n/a
Catalog #	Kit-2109
Price
Order / More Info	Uridine diphospho-glucuronosyltransferases Activity Assay / Ligand Screening Kit from CREATIVE BIOMART
Product Specific References	n/a
產(chǎn)品資料

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