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產(chǎn)品名稱

MutaGEL? Aldolase B (24) from EAGLE BIOSCIENCES INC.

產(chǎn)品貨號

KE09013

產(chǎn)品價格

現(xiàn)貨詢價，電話：010-67529703

產(chǎn)品規(guī)格

24 samples

產(chǎn)品品牌

EAGLE BIOSCIENCES INC.

產(chǎn)品概述

產(chǎn)品詳情

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Product Name	MutaGEL? Aldolase B (24)
Description	The Eagle Biosciences MutaGEL? Aldolase B (AS) is an amplification refractionary mutation system (ARMS) containing two sets of primers for both allelspecific sequences within the fructaldolase gene. The sequence specific primers can be used directly for PCR with extracted DNA. The resulting amplification products are subsequently identified with gelelectrophoretic methods. If there is no specific allel product detectable, the correct PCR procedure is proved by an internal control. The present genotype of unknown samples is interpreted by detection of corresponding DNA-amplificates for normal (G) - and mutation (C) - constellation in two separate lanes of the gel (method by Dr. Essrich, Biologisches Labor, Denzlingen). Sample Types: DNA. ? The liver isoenzyme Aldolase B is critical for sugar metabolism, and a catalytic deficiency due to mutations in its gene may result in hereditary fructose intolerance (HFI) syndrome, with hypoglycaemia and severe abdominal symptoms.The autosomal recessive disorder HFI is a potentially lethal inborn error in metabolism and the disease poses diagnostic problems because of in part very unspecific clinical manifestations. The present aldolase B PCR test is useful for the detection of the three most common mutations critical for gluconeogenesis and fructose metabolism: A149P (60%), A174D (11%) and N334K (8%). These mutations may account together for more than 80% of all known mutations causing HFI and their screening will be helpful for suited therapy of afflicted patients.The G>C mutation at bp position 448 results in an amino-acid exchange (from alanine to praline) at position 150 (earlier 149). Frequency of that mutation is 1:250 in Caucasians and disease by homozygous presence of C-allel is about 1:50000 (=0.002%).
Size	24 samples
Concentration	n/a
Applications	RUO
Other Names	Aldolase B
Gene, Accession, CAS #	n/a
Catalog #	KE09013
Price
Order / More Info	MutaGEL® Aldolase B (24) from EAGLE BIOSCIENCES INC.
Product Specific References	n/a
產(chǎn)品資料

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