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產(chǎn)品名稱

Nitric Oxide Synthase Non-Enzymatic Assay Kit from EAGLE BIOSCIENCES INC.

產(chǎn)品貨號

NOS39-K01

產(chǎn)品價格

現(xiàn)貨詢價，電話：010-67529703

產(chǎn)品規(guī)格

1 x 96 well

產(chǎn)品品牌

EAGLE BIOSCIENCES INC.

產(chǎn)品概述

產(chǎn)品詳情

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Product Name	Nitric Oxide Synthase Non-Enzymatic Assay Kit
Description	In aqueous solution, nitric oxide rapidly degrades to nitrate and nitrite. Spectrophotometric quantitation of nitrite using Griess Reagent is straightforward, but does not measure nitrate. This kit employs metallic cadmium for quantitative conversion of nitrate to nitrite (figure 1) prior to quantitation of nitrate using Griess reagent - thus providing for accurate determination of total NO production. Sample Types: biological fluids. Range: 0.5 - 100 μM. Sensitivity: 1 pmol/μL or ~1 μM. Time: 5 minutes ? Nitric oxide can be spectrophotometrically assayed by measuring the accumulation of its stable degradation products, nitrate and nitrite. The ratio of these two products in biological fluids, tissue culture media, etc. may vary substantially. Hence, for accurate assessment of the total nitric oxide generated, one must monitor both nitrate and nitrite. An excellent solution to this problem involves the conversion of nitrate to nitrite catalyzed by cadmium. This is then followed by the quantitation of nitrite using the griess reaction. This kit is suitable for the quantitative determination of total nitric oxide in samples that have high protein concentrations. The cadmium catalyst proves far more robust when in the presence of harsh deproteinating reagents than the alternative, nitrate reductase.
Size	1 x 96 well
Concentration	n/a
Applications	RUO
Other Names	Nitric Oxide Synthase, NOS, NADPH, Arginine, Citrulline
Gene, Accession, CAS #	n/a
Catalog #	NOS39-K01
Price
Order / More Info	Nitric Oxide Synthase Non-Enzymatic Assay Kit from EAGLE BIOSCIENCES INC.
Product Specific References	n/a
產(chǎn)品資料

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