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| Antigenic Specificity | 6X His Tag |
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| Clone | 33D10.D2 |
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| Host Species | Mouse |
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| Reactive Species | n/a |
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| Isotype | IgG1 kappa |
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| Format | DyLight? 549 conjugate |
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| Size | 100 μg |
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| Concentration | 1.0 mg/mL |
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| Applications | FLISA, IF Microscopy, Western Blot. The emission spectra for this DyLight? conjugate match the principle output wavelengths of most common fluorescence instrumentation. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | This protein-A purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells. |
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| Immunogen | This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide. |
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| Other Names | mouse anti-6X His Tag DyLight? 549 conjugated Antibody, DyLight? 549 conjugated mouse anti-6X His Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody |
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| Gene, Accession # | n/a |
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| Catalog # | 200-342-382 |
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| Price | |
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| Order / More Info | 6X His Tag Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | Brandsma, M ; X Wang, H Diao, SE. Kohalmi1, AM Jevnikar and S. Ma (2009) A Proficient Approach to the Production of Therapeutic Glucagon-Like Peptide-1 (GLP-1) in Transgenic PlantsThe Open Biotechnology Journal, 3, 57-66 57 1874-0707/09 |
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